Heider J, Leinfelder W, Böck A
Lehrstuhl für Mikrobiologie, Universität München, FRG.
Nucleic Acids Res. 1989 Apr 11;17(7):2529-40. doi: 10.1093/nar/17.7.2529.
The selC gene from E. coli codes for a tRNA species (tRNA(UCASer] which is aminoacylated with L-serine and which cotranslationally inserts selenocysteine into selenoproteins. By means of Southern hybridization it was demonstrated that this gene occurs in all enterobacteria tested. To assess whether the unique primary and secondary structural features of the E. coli selC gene product are conserved in that of other organisms, the selC homologue from Proteus vulgaris was cloned and sequenced. It was found that the Proteus selC gene differs from the E. coli counterpart in only six nucleotides, that it displays the same unique properties and that it is expressed and functions in E. coli. This indicates that the unique mechanism of selenocysteine incorporation is not restricted to E. coli but has been conserved as a uniform biochemical process.
来自大肠杆菌的selC基因编码一种tRNA（tRNA[UCASer]），它被L-丝氨酸氨酰化，并在共翻译过程中将硒代半胱氨酸插入硒蛋白中。通过Southern杂交证明，该基因存在于所有测试的肠杆菌中。为了评估大肠杆菌selC基因产物独特的一级和二级结构特征在其他生物体中是否保守，对普通变形杆菌的selC同源物进行了克隆和测序。发现变形杆菌的selC基因与大肠杆菌的对应基因仅在六个核苷酸上不同，它具有相同的独特性质，并且在大肠杆菌中表达并发挥功能。这表明硒代半胱氨酸掺入的独特机制不仅限于大肠杆菌，而是作为一种统一的生化过程被保守下来。
