Ronner A C, Curtin J, Karami N, Ronner U
ABIGO Medical AB, Sahlgrenska Science Park, Gothenburg, Sweden.
J Wound Care. 2014 Oct;23(10):484, 486-8. doi: 10.12968/jowc.2014.23.10.484.
The aim of this in vitro study was to demonstrate the binding capacity of multiple meticillin-resistant Staphylococcus aureus (MRSA) strains and compare the binding capacity to meticillin-sensitive Staphylococcus aureus.
The binding of Staphylococcus aureus to a surface was assessed by bioluminescent monitoring of the bacterial ATP levels. This assay can be used as an in vitro diagnostic model for bacteria binding in a critically colonised wound.
Eleven strains of Staphylococcus aureus were examined including MRSA, all of which efficiently and equally adhered to the dialkyl carbamoyl chloride (DACC)-coated dressing (Sorbact; Abigo Medical AB). The binding capacity was all in the same range 0.7-2.9 × 10⁶ CFU/cm². regardless of the antibiotic resistance properties of the specific strain.
The decrease of wound bioburden of Staphylococcus aureus including MRSA is the result of the high binding capacity shown in this study and by earlier data. The findings in this study strengthen the held view that development of antibiotic resistance has minimal impact on the surface structures of the microorganisms in wounds.
本体外研究旨在证明多种耐甲氧西林金黄色葡萄球菌（MRSA）菌株的结合能力，并比较其与甲氧西林敏感金黄色葡萄球菌的结合能力。
通过对细菌ATP水平进行生物发光监测来评估金黄色葡萄球菌与表面的结合情况。该检测可作为严重定植伤口中细菌结合的体外诊断模型。
检测了包括MRSA在内的11株金黄色葡萄球菌，所有菌株均能高效且同等程度地黏附于二烷基氨基甲酰氯（DACC）包被的敷料（Sorbact；阿比戈医疗公司）上。结合能力均在0.7 - 2.9×10⁶ CFU/cm²的相同范围内，与特定菌株的抗生素耐药特性无关。
包括MRSA在内的金黄色葡萄球菌伤口生物负荷的降低是本研究及早期数据所显示的高结合能力的结果。本研究结果强化了以下观点，即抗生素耐药性的发展对伤口中微生物的表面结构影响极小。
