Cooper R, Jenkins L
At Centre for Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB.
J Wound Care. 2016 Feb;25(2):76, 78-82. doi: 10.12968/jowc.2016.25.2.76.
To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro.
Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm.
Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples.
Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm.
This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
迄今为止，仅发现浮游细菌能不可逆地结合于二烷基氨基甲酰氯（DACC）包被的Cutimed Sorbact敷料。因此，本研究旨在确定细菌生物膜在体外是否能结合于DACC包被的敷料。
将DACC包被的敷料样本和未包被的对照敷料样本（由英国赫尔的BSN医疗有限公司提供）与已培养铜绿假单胞菌或耐甲氧西林金黄色葡萄球菌（MRSA）生物膜24小时的塑料盖玻片接触。通过扫描电子显微镜检查敷料样本，以检测生物膜的存在。
铜绿假单胞菌生物膜能强烈结合于DACC包被的和未包被的敷料样本。MRSA结合于DACC包被的敷料比未包被的样本更广泛。
两种不同测试细菌的生物膜在体外能结合于敷料，且敷料上的DACC包被增强了MRSA生物膜的结合。
本研究由英国赫尔的BSN医疗有限公司资助。该公司对实验设计或结果解释没有影响。
