Sun Juan, Rong Zhang, Wugeti Najina, Azhati Adila, GUo Yujun, Liu Haili, Qian Ruping, Zhao Long, Ma Yitong
Clinical Postdoctoral Research Station, The First Affiliated Hospital of Xinjiang Medical University, Urumuqi, China (mainland).
Function Center, Xinjiang Medical University, Urumuqi, China (mainland).
Med Sci Monit. 2014 Oct 9;20:1884-9. doi: 10.12659/MSM.891242.
The aim of this study was the establishment of a rapid atrial pacing (RAP)-induced atrial fibrillation (AF) model with electrophotoluminescence and the application of ultrasonic backscatter quantitative analysis of the degree of myocardial fibrosis in New Zealand white rabbits.
Sixteen New Zealand white rabbits were randomly divided into 2 groups: 1) a sham operation group (n=8) with implanted electrodes and no rapid pacing and 2) a pacing group (n=8) with an AF model induced by short-term rapid right atrial pacing for 12 h. Establishment of an AF model, atrial myocardium of myocardial fibrosis was tested by Masson staining and expression of collagen I and collagen III protein was detected with pathologic immunohistochemistry integrated back-scatter (IBS). Back scattering integral cycle variation (CVIB) were detected in atrial septal and posterior wall of the right atrium.
Rapid atrial pacing successfully induced the atrial fibrillation model in rabbits. Masson staining showed myocardial fibrosis significantly increased in the pacing group. Expression of collagen I and collagen III protein was strongly positive in the pacing group, and expression of collagen I and collagen III protein were weakly positive in the sham operation group. Compared with the sham operation group, AII was increased (8.24±0.85 vs. 15.56±1.30, P<0.05) and (7.58±0.56 vs. 16.60±2.45, P<0.05). CVIB was significantly decreased (2.78±0.86 vs. 1.08±0.13, P<0.05) and (3.12±0.65 vs. 1.56±0.15, P<0.05) in septal and posterior wall of the right atrium of the pacing group.
Ultrasonic backscatter measurement technique can be used to evaluate degree of myocardial fibrosis in a right atrial pacing-induced atrial arrhythmia model.
本研究旨在建立一种利用电光发光技术的快速心房起搏（RAP）诱导心房颤动（AF）模型，并应用超声背向散射定量分析新西兰白兔心肌纤维化程度。
16只新西兰白兔随机分为2组：1）假手术组（n = 8），植入电极但无快速起搏；2）起搏组（n = 8），通过短期快速右心房起搏12小时诱导AF模型。建立AF模型后，采用Masson染色检测心房心肌纤维化，并用病理免疫组织化学积分背向散射（IBS）检测I型和III型胶原蛋白的表达。在房间隔和右心房后壁检测背向散射积分周期变化（CVIB）。
快速心房起搏成功诱导兔心房颤动模型。Masson染色显示起搏组心肌纤维化显著增加。起搏组I型和III型胶原蛋白表达呈强阳性，假手术组I型和III型胶原蛋白表达呈弱阳性。与假手术组相比，起搏组房间隔和右心房后壁的AII增加（8.24±0.85 vs. 15.56±1.30，P<0.05）和（7.58±0.56 vs. 16.60±2.45，P<0.05）。CVIB显著降低（2.78±0.86 vs. 1.08±0.13，P<0.05）和（3.12±0.65 vs. 1.56±0.15，P<0.05）。
超声背向散射测量技术可用于评估右心房起搏诱导的房性心律失常模型中的心肌纤维化程度。
