Farooq U, Cho S, Rybnik-Trzaskowska P K, Singh R P, Malecki I A
School of Animal Biology M085, Faculty of Science, The University of Western Australia, Crawley, Australia; The UWA Institute of Agriculture M082, The University of Western Australia, Crawley, Australia; Department of Poultry Science, University of Agriculture, Faisalabad, Pakistan.
School of Animal Biology M085, Faculty of Science, The University of Western Australia, Crawley, Australia; The UWA Institute of Agriculture M082, The University of Western Australia, Crawley, Australia.
Theriogenology. 2015 Jan 15;83(2):162-7. doi: 10.1016/j.theriogenology.2014.09.006. Epub 2014 Sep 16.
The proctodeal gland of the male Japanese quail produces thick foam that accompanies semen when it is transferred to the female. It is thought that this foam enhances fertilization by improving the motility of the sperm, but reports are conflicting because the effect of foam on sperm motility has only been assessed subjectively The velocity of individual sperm was not able to be measured accurately, variations were large, and small changes in motility could not be accurately evaluated. So, we tested the hypothesis that foam affects the motility of spermatozoa of Japanese quail by analyzing motility objectively using computer-assisted semen analysis and determining changes in sperm kinetics in the presence of different concentrations of proctodeal gland foam. The addition of 5% or 10% foam to the sperm suspension increased (P < 0.05) all sperm kinetic parameters (the curvilinear velocity, straight line velocity, the velocity of the average path, linearity, straightness, and beat cross frequency). As a result, the percentage of motile and progressive motile sperm also increased. All these parameters declined (P < 0.05) with a further increase in the concentration of foam to 15% and 20%. Furthermore, this effect was similar in males that were 8, 16, or 26 weeks of age. We conclude that sperm motility is enhanced by proctodeal gland foam, and this enhancement depends on its concentration.
雄性日本鹌鹑的泄殖腔腺会产生浓稠的泡沫，在精液传输至雌性体内时，该泡沫会伴随精液一同排出。据认为，这种泡沫通过提高精子的活力来增强受精能力，但相关报告存在矛盾之处，因为对泡沫对精子活力的影响仅进行了主观评估。单个精子的速度无法准确测量，变化幅度较大，活力的微小变化也无法准确评估。因此，我们通过使用计算机辅助精液分析客观地分析精子活力，并确定在不同浓度的泄殖腔腺泡沫存在下精子动力学的变化，来检验泡沫会影响日本鹌鹑精子活力这一假设。向精子悬浮液中添加5%或10%的泡沫会使所有精子动力学参数（曲线速度、直线速度、平均路径速度、线性度、直线性和鞭打交叉频率）增加（P < 0.05）。结果，有活力和进行性运动精子的百分比也增加了。随着泡沫浓度进一步增加至15%和20%，所有这些参数均下降（P < 0.05）。此外，在8周龄、16周龄或26周龄的雄性鹌鹑中，这种效应是相似的。我们得出结论，泄殖腔腺泡沫可增强精子活力，且这种增强作用取决于其浓度。
