鱿鱼皮硫酸多糖：潜在的生物学活性及部分纯化。
Sulfated polysaccharides from Loligo vulgaris skin: potential biological activities and partial purification.
出版信息
Int J Biol Macromol. 2015 Jan;72:1143-51. doi: 10.1016/j.ijbiomac.2014.09.041.
The characteristics, biological properties, and purification of sulfated polysaccharides extracted from squid (Loligo vulgaris) skin were investigated. Their chemical and physical characteristics were determined using X-ray diffraction and infrared spectroscopic analysis. Sulfated polysaccharides from squid skin (SPSS) contained 85.06% sugar, 2.54% protein, 1.87% ash, 8.07% sulfate, and 1.72% uronic acid. The antioxidant properties of SPSS were investigated based on DPPH radical-scavenging capacity (IC50 = 19.42 mg mL(-1)), hydrogen peroxide-scavenging activity (IC50 = 0.91 mg mL(-1)), and β-carotene bleaching inhibition (IC50 = 2.79 mg mL(-1)) assays. ACE-inhibitory activity of SPSS was also investigated (IC50 = 0.14 mg mL(-1)). Further antimicrobial activity assays indicated that SPSS exhibited marked inhibitory activity against the bacterial and fungal strains tested. Those polysaccharides did not display hemolytic activity towards bovine erythrocytes. Fractionation by DEAE-cellulose column chromatography showed three major absorbance peaks. Results of this study suggest that sulfated polysaccharides from squid skin are attractive sources of polysaccharides and promising candidates for future application as dietary ingredients.
研究了鱿鱼（Loligo vulgaris）皮中提取的硫酸化多糖的特性、生物学特性和纯化。通过 X 射线衍射和红外光谱分析确定了它们的化学和物理特性。鱿鱼皮硫酸化多糖（SPSS）含有 85.06%的糖、2.54%的蛋白质、1.87%的灰分、8.07%的硫酸盐和 1.72%的糖醛酸。基于 DPPH 自由基清除能力（IC50 = 19.42 mg mL(-1))、过氧化氢清除活性（IC50 = 0.91 mg mL(-1))和 β-胡萝卜素漂白抑制（IC50 = 2.79 mg mL(-1))测定，研究了 SPSS 的抗氧化性能。还研究了 SPSS 的 ACE 抑制活性（IC50 = 0.14 mg mL(-1))。进一步的抗菌活性测定表明，SPSS 对测试的细菌和真菌菌株表现出显著的抑制活性。这些多糖对牛红细胞没有溶血活性。DEAE-纤维素柱层析分离显示出三个主要的吸收峰。本研究结果表明，鱿鱼皮硫酸化多糖是有吸引力的多糖来源，是未来作为膳食成分应用的有前途的候选物。
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