Sottiurai V S, Sue S L, Hsu M K, Mann W K, Batson R C
Department of Surgery, Louisiana State University School of Medicine, New Orleans.
J Cardiovasc Surg (Torino). 1989 Sep-Oct;30(5):808-16.
Twelve specially designed crimped aortobifurcated grafts (aorta, woven 200 porosity; right limb, woven 500 porosity; left limb, knitted 1200 porosity) were implanted into mongrel dogs that were sacrificed at two, six, eight and twelve-month intervals. Pseudointima from the mid portion of each type of graft was studied with light (LM) and transmission electron microscopy (TEM). An ocular micrometer was used to determine the thickness of the pseudointima. A trilaminar cytoarchitecture resembling the wall of a medium-sized artery existed in the pseudointima. This was characterized by an inner cellular layer, outer fibrocollagenous layer, and multi-interlamination of cells and glycosaminoglycan in the middle layer. The cellular inner layer was thickest in the knitted and thinnest in the woven 200 porosity. A zone of acellularity separated the luminal cells from the underlying myoblasts and myofibroblasts. Specificity of varying types of cell distribution occurred in the pseudointima. Myoblasts and myofibroblasts were located near the lumen while fibroblasts and mesenchymoid cells were situated near the graft. The luminal cells had the combined features of endothelial cells and myofibroblasts. Collagen and glycosaminoglycans were the dominant extracellular matrix. Despite the difference of fluid permeability in high porosity woven and knitted dacron grafts, the pseudointima was well formed and revealed no striking general cytoarchitectural difference between these two types of grafts.
将十二个特别设计的卷曲型主动脉分叉移植物（主动脉，编织而成，孔隙率为200；右支，编织而成，孔隙率为500；左支，针织而成，孔隙率为1200）植入杂种犬体内，分别在2个月、6个月、8个月和12个月的间隔期对犬实施安乐死。对每种移植物中部的假内膜进行光镜（LM）和透射电子显微镜（TEM）研究。使用目镜测微计确定假内膜的厚度。假内膜中存在一种类似于中等大小动脉壁的三层细胞结构。其特征为内层细胞层、外层纤维胶原层以及中间层细胞与糖胺聚糖的多层交织。细胞内层在针织移植物中最厚，在孔隙率为200的编织移植物中最薄。无细胞区将管腔细胞与下方的成肌细胞和肌成纤维细胞分隔开来。假内膜中不同类型细胞分布具有特异性。成肌细胞和肌成纤维细胞位于管腔附近，而成纤维细胞和间充质样细胞位于移植物附近。管腔细胞具有内皮细胞和肌成纤维细胞的综合特征。胶原蛋白和糖胺聚糖是主要的细胞外基质。尽管高孔隙率编织和针织涤纶移植物在流体渗透性方面存在差异，但假内膜形成良好，且这两种类型的移植物在细胞结构上未显示出明显的总体差异。
