Sottiurai V S, Sue S L, Rau D J, Tran A B
Department of Surgery, Louisiana State University School of Medicine, New Orleans.
J Cardiovasc Surg (Torino). 1989 Nov-Dec;30(6):902-9.
Twenty-one grafts (knitted crimped, 7; knitted Gelseal, 7; knitted no crimp, 7) of 7 cm long were inserted into 6 month-old mongrel puppies as infrarenal aortic tube grafts and retrieved at two-month intervals. All grafts were perfusion fixed in situ. Ex vivo arteriograms were obtained prior to processing for light (LM) and transmission electron microscopic (TEM) studies of tissue ingrowth into the grafts. All grafts were patent at the time of harvesting. The thickness of pseudointima plateaued at 10 weeks. There was no demonstrable discrepancy in thickness between grafts (cell 72 +/- 13). The luminal cells were modified myofibroblasts that contained short microvilli, gap junctions, myofilament, and incomplete basal laminae. The pseudointima consisted of an interlamination of myofibroblasts alternating with extracellular matrix of collagen and ground substances. It was more cellular near the lumen and more fibrocollagenous near the graft. Myofibroblasts were found near the lumen and fibroblasts near the graft. Macrophages, vasa vasorum, leukocytes and fibroblasts occupied the interstitial space between the graft fibrils. Similar cellular and extracellular composition existed in the three types of grafts. There was no local inflammatory reaction encountered in the Gelseal treated graft. Gelseal, crimped and noncrimped knitted Dacron grafts had pseudointima of comparable architecture, thickness, cellular and noncellular composition. Gelseal did not hinder pseudointima formation or induce local inflammatory reaction.
将21根7厘米长的移植物（针织卷曲型7根、针织Gelseal型7根、针织无卷曲型7根）作为肾下腹主动脉血管移植物植入6个月大的杂种幼犬体内，并每隔两个月取出。所有移植物均在原位进行灌注固定。在对移植物内组织生长进行光镜（LM）和透射电子显微镜（TEM）研究处理之前，获得离体血管造影照片。所有移植物在收获时均保持通畅。假内膜厚度在10周时趋于稳定。各移植物之间在厚度上没有明显差异（细胞数72±13）。腔内细胞为改良的肌成纤维细胞，含有短微绒毛、缝隙连接、肌丝和不完整的基膜。假内膜由肌成纤维细胞与胶原蛋白和基质的细胞外基质交替分层组成。靠近管腔处细胞较多，靠近移植物处纤维胶原较多。在管腔附近发现肌成纤维细胞，在移植物附近发现成纤维细胞。巨噬细胞、血管滋养管、白细胞和成纤维细胞占据了移植物纤维之间的间隙。三种类型的移植物中存在相似的细胞和细胞外成分。在Gelseal处理的移植物中未发现局部炎症反应。Gelseal、卷曲型和非卷曲型针织涤纶移植物具有结构、厚度、细胞和非细胞成分相当的假内膜。Gelseal不阻碍假内膜形成或诱导局部炎症反应。
