Van Rooijen N
Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
J Immunol Methods. 1989 Nov 13;124(1):1-6. doi: 10.1016/0022-1759(89)90178-6.
Macrophages form an important cell population involved in numerous immunological processes. Techniques which eliminate these cells, in order to permit 'in vivo' studies of their function are generally imperfect and for that reason we have developed a method using the liposome encapsulated drug dichloromethylene diphosphonate (Cl2MDP). The liposomes are ingested by the macrophages which are then destroyed following phospholipase-mediated disruption of the liposomal bilayers and release of the Cl2MDP. In the present review, technical details of the preparation of liposome encapsulated Cl2MDP are given, and applications of the technique are discussed.
巨噬细胞是参与众多免疫过程的重要细胞群体。为了对其功能进行“体内”研究而消除这些细胞的技术通常并不完善，因此我们开发了一种使用脂质体包裹药物二氯亚甲基二膦酸盐（Cl2MDP）的方法。巨噬细胞摄取脂质体，随后在磷脂酶介导的脂质体双层破坏和Cl2MDP释放后被破坏。在本综述中，给出了脂质体包裹Cl2MDP制备的技术细节，并讨论了该技术的应用。
