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在小鼠T细胞受体β2基因座中鉴定出一个功能性核因子κB结合位点。

Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus.

作者信息

Jamieson C, Mauxion F, Sen R

机构信息

Department of Biology, Brandeis University, Waltham, Massachusetts 02254.

出版信息

J Exp Med. 1989 Nov 1;170(5):1737-43. doi: 10.1084/jem.170.5.1737.

DOI:10.1084/jem.170.5.1737
PMID:2530301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189503/
Abstract

We have identified a sequence in the TCR beta 2 locus that is homologous to the kappa B site in the Ig kappa light chain enhancer. This element, TCR beta-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragment encompassing this site is preferentially active in T cells stimulated with phorbol esters or the HTLV-1 tax gene product compared with a B cell line that constitutively expresses NF-kappa B. Our results provide the first evidence for transcriptional regulatory sequences residing within the J beta 2-C beta 2 intron and suggest the possible involvement of these sequences in modulation of TCR beta gene expression upon cellular activation.

摘要

我们在TCRβ2基因座中鉴定出一段序列，它与Igκ轻链增强子中的κB位点同源。这个元件，即TCRβ-B，位于先前鉴定的T细胞特异性DNase1超敏位点附近。转染分析表明，与组成性表达NF-κB的B细胞系相比，包含该位点的60bp片段在佛波酯或HTLV-1 tax基因产物刺激的T细胞中具有优先活性。我们的结果为Jβ2-Cβ2内含子中的转录调控序列提供了首个证据，并表明这些序列可能参与细胞活化后TCRβ基因表达的调节。

相似文献

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Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus.在小鼠T细胞受体β2基因座中鉴定出一个功能性核因子κB结合位点。
J Exp Med. 1989 Nov 1;170(5):1737-43. doi: 10.1084/jem.170.5.1737.
2
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The kappa B transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study.κB转录增强子基序和V(D)J重组的信号序列是锌指蛋白HIVEP3/KRC的靶点：一项位点选择扩增结合研究。
BMC Immunol. 2002 Aug 22;3:10. doi: 10.1186/1471-2172-3-10.
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NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.核因子-κB RelA缺陷淋巴细胞：T细胞和B细胞正常发育，IgA和IgG1产生受损，增殖反应降低。
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Identification of a rel-related protein in the nucleus during the S phase of the cell cycle.在细胞周期的S期期间，在细胞核中鉴定出一种与Rel相关的蛋白质。
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