Bolechová Martina, Cáslavský Josef, Pospíchalová Markéta, Kosubová Petra
Central Institute for Supervising and Testing in Agriculture, Hroznová 2, Brno 656 06, Czech Republic; Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, Brno 612 00, Czech Republic.
Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, Brno 612 00, Czech Republic.
Food Chem. 2015 Mar 1;170:265-70. doi: 10.1016/j.foodchem.2014.08.072. Epub 2014 Aug 26.
Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow these dangerous plant toxins to be determined. In this study, a fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Sample preparation was based on a modified QuEChERS approach. The mean recovery, precision, matrix effects and limits of quantification were assessed for three matrices within the method validation. The presented method was used to inspect 41 various feed samples, where the presence of PAs was expected. Roughages and feed for rabbits contained the highest levels of PAs, in general.
被称为次生代谢产物的生物碱根据典型的结构特征被归类为大家族，如包含350多种单个杂环化合物的吡咯里西啶生物碱（PAs）。PAs对人类和牲畜构成严重的健康风险；因此，需要有能够测定这些危险植物毒素的方法。在本研究中，提出了一种快速、可靠且灵敏的方法来鉴定和定量饲料样品中的PAs。通过超高效液相色谱-串联质谱法测定了包括野百合碱、千里光裂碱、千里光碱、西洋千里光碱和倒千里光碱在内的PAs。样品制备基于改良的QuEChERS方法。在方法验证过程中评估了三种基质的平均回收率、精密度、基质效应和定量限。所提出的方法用于检测41种预期含有PAs的不同饲料样品。一般来说，粗饲料和兔饲料中的PAs含量最高。
