The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Agilent Technologies Co. Ltd. (China), No.3, Wang Jing Bei Road, Chao Yang District, Beijing 100102, China.
Molecules. 2019 Mar 19;24(6):1077. doi: 10.3390/molecules24061077.
Pyrrolizidine alkaloids (PAs) are natural toxins found in some genera of the family Asteraceae. However, it has not been reported whether PAs are present in the widely used Asteraceae plant Thunb. (). The purpose of this study was to establish a sensitive and rapid UPLC-MS/MS method together with chemometrics analysis for simultaneous determination and risk assessment of PAs in . The developed UPLC-MS/MS method was validated and was confirmed to display desirable high selectivity, precision and accuracy. Risk assessment was conducted according to the European Medicines Agency (EMA) guideline. Chemometrics analysis was performed with hierarchical clustering analysis and principal component analysis to characterize the differences between PAs of . Finally, PAs were found in 29 out of 30 samples and at least two were detected in each sample, besides, more than half of the samples exceeded the EMA baseline. Nevertheless, the chemometrics results suggested that the PAs contents of from different sources varied significantly. The method was successfully applied to the detection and risk evaluation of PAs-containing for the first time. This study should provide a meaningful reference for the rational and safe use of .
吡咯里西啶生物碱（PAs）是菊科某些属中发现的天然毒素。然而，目前还没有报道称广泛使用的菊科植物旋覆花（Thunb.）中是否存在 PAs。本研究旨在建立一种灵敏快速的 UPLC-MS/MS 方法，并结合化学计量学分析，同时对旋覆花中的 PAs 进行定量和风险评估。所建立的 UPLC-MS/MS 方法经过验证，具有良好的选择性、精密度和准确度。风险评估按照欧洲药品管理局（EMA）指南进行。化学计量学分析采用层次聚类分析和主成分分析，以表征旋覆花中 PAs 的差异。最后，在 30 个样本中的 29 个样本中发现了 PAs，每个样本中至少检测到两种，此外，超过一半的样本超过了 EMA 基线。然而，化学计量学结果表明，不同来源的旋覆花中的 PAs 含量差异显著。该方法首次成功应用于含 PAs 的旋覆花的检测和风险评估。本研究为旋覆花的合理安全使用提供了有意义的参考。
