Syed Haroon Khalid, Liew Kai Bin, Loh Gabriel Onn Kit, Peh Kok Khiang
Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
Food Chem. 2015 Mar 1;170:321-6. doi: 10.1016/j.foodchem.2014.08.066. Epub 2014 Aug 26.
A stability-indicating HPLC-UV method for the determination of curcumin in Curcuma longa extract and emulsion was developed. The system suitability parameters, theoretical plates (N), tailing factor (T), capacity factor (K'), height equivalent of a theoretical plate (H) and resolution (Rs) were calculated. Stress degradation studies (acid, base, oxidation, heat and UV light) of curcumin were performed in emulsion. It was found that N>6500, T<1.1, K' was 2.68-3.75, HETP about 37 and Rs was 1.8. The method was linear from 2 to 200 μg/mL with a correlation coefficient of 0.9998. The intra-day precision and accuracy for curcumin were ⩽0.87% and ⩽2.0%, while the inter-day precision and accuracy values were ⩽2.1% and ⩽-1.92. Curcumin degraded in emulsion under acid, alkali and UV light. In conclusion, the stability-indicating method could be employed to determine curcumin in bulk and emulsions.
建立了一种用于测定姜黄提取物和乳剂中姜黄素的稳定性指示高效液相色谱 - 紫外检测法。计算了系统适用性参数，包括理论塔板数（N）、拖尾因子（T）、容量因子（K'）、理论塔板高度（H）和分离度（Rs）。在乳剂中对姜黄素进行了强制降解研究（酸、碱、氧化、加热和紫外光）。结果发现N>6500，T<1.1，K'为2.68 - 3.75，理论塔板高度约为37，Rs为1.8。该方法在2至200μg/mL范围内呈线性，相关系数为0.9998。姜黄素的日内精密度和准确度分别≤0.87%和≤2.0%，而日间精密度和准确度值分别≤2.1%和≤ - 1.92。姜黄素在乳剂中在酸、碱和紫外光条件下会降解。总之，该稳定性指示方法可用于测定原料药和乳剂中的姜黄素。
