China Military Institute of Chinese Materia Medica, 302 Military Hospital, Beijing 100039, China.
J Pharm Biomed Anal. 2010 Sep 21;53(1):43-9. doi: 10.1016/j.jpba.2010.03.021. Epub 2010 Mar 27.
A new reversed phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of three curcuminoids (curcumin (C), desmethoxycurcumin (DMC) and bisdesmethoxycurcumin (BDMC)) in Curcuma longa Linn. (C. longa) using a Waters BEH Shield RP C(18), 2.1 mm x 100 mm, 1.7 microm column. The runtime was 2 min. The influence of column temperature and mobile phase on resolution was investigated. The method was validated according to the ICH guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The limits of detection were 40.66, 49.38 and 29.28 pg for C, DMC and BDMC, respectively. Limits of quantitation for C, DMC and BDMC, were 134.18, 164.44 and 97.50 pg, respectively. Linear range was from 3.28 to 46.08 microg/ml. The mean+/-SD percent recoveries of curcuminoids were 99.47+/-1.66, 99.50+/-1.99 and 97.77+/-2.37 of C, DMC and BDMC, respectively. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The proposed method was found to be reproducible and convenient for quantitative analysis of three curcuminoids in C. longa. This work provided some references for quality control of C. longa.
建立了一种新的反相超高效液相色谱（UPLC）方法，用于快速定量分析姜黄中的三种姜黄素（姜黄素（C）、脱甲氧基姜黄素（DMC）和双脱甲氧基姜黄素（BDMC））。采用 Waters BEH Shield RP C（18），2.1mm×100mm，1.7μm 柱，运行时间为 2min。考察了柱温和流动相对分离度的影响。该方法按照 ICH 分析程序验证指南进行了验证，包括精密度、准确度和线性度。C、DMC 和 BDMC 的检测限分别为 40.66、49.38 和 29.28pg。C、DMC 和 BDMC 的定量限分别为 134.18、164.44 和 97.50pg。线性范围为 3.28 至 46.08μg/ml。姜黄素的平均+/-SD 回收率分别为 99.47+/-1.66%、99.50+/-1.99%和 97.77+/-2.37%。与传统 HPLC 相比，该方法在分析时间、效率和灵敏度方面具有优势。该方法重复性好，可用于定量分析姜黄中的三种姜黄素，为姜黄的质量控制提供了参考。
