利用超临界流体色谱-质谱联用技术直接检测食用油中的游离脂肪酸。
Direct detection of free fatty acids in edible oils using supercritical fluid chromatography coupled with mass spectrometry.
机构信息
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
出版信息
Food Chem. 2015 Mar 1;170:463-9. doi: 10.1016/j.foodchem.2014.08.043. Epub 2014 Aug 20.
Determination of free fatty acids (FFAs) in food products is of enormous interest mainly because they are related to the quality and authenticity of the oils. In this study, supercritical fluid chromatography (SFC), followed by an electrospray ionisation triple-quadrupole mass spectrometry (ESI-MS), is shown to provide a novel method for the separation and detection of FFAs in edible oils without any pretreatment. Eight FFAs were separated on a HSS C18 SB column with gradient elution within 3 min. Effects of different columns, modifiers and column temperature changes were evaluated. The results indicated the feasibility of this method for the high-throughput determination of individual FFAs with satisfactory correlation coefficients (R(2)>0.994) and good reproducibility of RSD<13.5% (intraday) and <15.0% (interday). By combined with principal component analysis (PCA), different types of edible oil were successfully distinguished into several categories, showing a potential application for the determination of oil quality or authenticity.
测定食品中的游离脂肪酸（FFAs）具有重要意义，因为它们与油脂的质量和真实性有关。本研究采用超临界流体色谱（SFC），随后采用电喷雾离子化三重四极杆质谱（ESI-MS），展示了一种无需任何预处理即可分离和检测食用油中 FFAs 的新方法。八种 FFAs 在 HSS C18 SB 柱上用梯度洗脱在 3 分钟内分离。评价了不同色谱柱、改性剂和柱温变化的影响。结果表明，该方法具有高通量测定单个 FFAs 的可行性，相关系数（R(2)>0.994）令人满意，日内和日间的重现性良好，RSD<13.5%和<15.0%。通过与主成分分析（PCA）相结合，不同类型的食用油成功地分为几类，显示出在确定油质或真实性方面的潜在应用。
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