Zhang Rong-Ping, Liu He-He, Li Qing-Qing, Wang Yan, Liu Jun-Ying, Hu Ji-Wei, Yan Xi-Ping, Gou Hua, Li Liang, Wang Ji-Wen
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China
Poult Sci. 2014 Dec;93(12):3104-11. doi: 10.3382/ps.2014-04145. Epub 2014 Oct 11.
In this study, we aimed to use duck breast muscle and leg muscle, the 2 main productive muscle organs, as a model to elucidate the molecular mechanism controlling how the 2 muscles have different deposition capabilities, and to analyze the mechanisms facilitating duck muscle development posthatching. Peking duck breast muscle and leg muscle were collected 3, 7, and 16 wk posthatching. The morphology of the myofibers was observed by paraffin sectioning the muscles. The expression of genes involved in protein metabolism [mammalian target of rapamycin (mTOR), RPS6-p70-protein kinase (S6K), forkhead box O1 (FoxO1), muscle RING finger 1 (MuRF1), and atrogin-1 (MAFbx)] was detected using real-time quantitative PCR and Western blot assays, and the results indicated that breast muscle had a stronger capacity for both protein synthesis and protein degradation compared with leg muscle. Satellite cell frequency declined during muscle development in both tissues, and the expression of Pax3/7, satellite cell marker genes, was not significantly different between breast muscle and leg muscle. No notable apoptosis was observed in either tissue. The results of this study suggest that protein metabolism signaling is the main reason promoting duck skeletal muscle mass gain.
在本研究中，我们旨在以鸭的胸肌和腿肌这两个主要的生产性肌肉器官为模型，阐明控制这两种肌肉具有不同沉积能力的分子机制，并分析促进雏鸭出壳后肌肉发育的机制。在出壳后3周、7周和16周采集北京鸭的胸肌和腿肌。通过对肌肉进行石蜡切片观察肌纤维的形态。使用实时定量PCR和蛋白质免疫印迹分析检测参与蛋白质代谢的基因[雷帕霉素靶蛋白（mTOR）、核糖体蛋白S6激酶（S6K）、叉头框蛋白O1（FoxO1）、肌肉环指蛋白1（MuRF1）和肌肉萎缩相关基因1（MAFbx）]的表达，结果表明，与腿肌相比，胸肌具有更强的蛋白质合成和蛋白质降解能力。在两个组织的肌肉发育过程中，卫星细胞频率均下降，且胸肌和腿肌之间卫星细胞标记基因Pax3/7的表达无显著差异。在任何一个组织中均未观察到明显的细胞凋亡。本研究结果表明，蛋白质代谢信号是促进鸭骨骼肌质量增加的主要原因。
