一种用于单分子研究的改进表面钝化方法。
An improved surface passivation method for single-molecule studies.
机构信息
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
1] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [2] Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. [3] Howard Hughes Medical Institute, Urbana, Illinois, USA.
出版信息
Nat Methods. 2014 Dec;11(12):1233-6. doi: 10.1038/nmeth.3143. Epub 2014 Oct 12.
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
我们报告了一种基于二氯二甲基硅烷（DDS）-吐温-20 的表面钝化方法，用于体外单分子研究，在测试条件下，与标准聚乙二醇（PEG）表面相比，它能更有效地防止生物分子的非特异性结合。DDS-吐温-20 表面制备简单、成本低廉，且不会干扰连接的生物分子的行为和活性。它还可用于在溶液中存在高浓度标记物种的情况下进行单分子成像。
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