Zhou Weiguang, Gao Shandian, Podgórska Katarzyna, Stadejek Tomasz, Qiu Hua-Ji, Yin Hong, Drew Trevor, Liu Lihong
Department of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden
Vaccine. 2014 Nov 20;32(49):6639-42. doi: 10.1016/j.vaccine.2014.09.058.
Classical swine fever (CSF), or hog cholera, is a highly contagious disease that emerged in the first half of the nineteenth century. To fight against the disease and protect pigs, different vaccines were developed, including early generation of lapinized Rovac strain and the later development of the “Chinese” strain (C-strain). However, details of the development of these vaccines are lost in history. In order to investigate the phylogenetic relationship between the Rovac and other lapinized vaccines, this study determined the genome sequence of the Rovac, which comprised 12,304 nucleotides, notably with the 3′untranslated region (3′UTR) containing a 13-nucleotide insertion. The near-complete genome of Russian vaccine strain LK-VNIVViM was determined by next-generation sequencing on Illumina MiSeq platform. Whole genome phylogenetic analysis revealed a closer relationship of the Rovac strain with the Russian LK-VNIVViM, CS strain and its derivative RUCSFPLUM (genotype 1.2), rather than with the C-strain (genotype 1.1). In addition, it demonstrated an ancestry role of the LK-VNIVViM in relation to the CS strain and RUCSFPLUM. The study suggested that the Rovac vaccine is the possible ancestor of the Russian vaccine strains but not the C-strain vaccine.
经典猪瘟（CSF），又称猪霍乱，是一种在19世纪上半叶出现的高度传染性疾病。为了对抗这种疾病并保护猪群，人们研发了不同的疫苗，包括早期的兔化Rovac株以及后来的“中国”株（C株）。然而，这些疫苗的研发细节已湮没在历史中。为了研究Rovac与其他兔化疫苗之间的系统发育关系，本研究测定了Rovac的基因组序列，其由12304个核苷酸组成，特别是3'非翻译区（3'UTR）包含一个13个核苷酸的插入片段。通过在Illumina MiSeq平台上进行二代测序，测定了俄罗斯疫苗株LK-VNIVViM的近乎完整的基因组。全基因组系统发育分析显示，Rovac株与俄罗斯的LK-VNIVViM、CS株及其衍生物RUCSFPLUM（基因型1.2）的关系更为密切，而与C株（基因型1.1）的关系较远。此外，它还证明了LK-VNIVViM相对于CS株和RUCSFPLUM的祖先作用。该研究表明，Rovac疫苗可能是俄罗斯疫苗株的祖先，而不是C株疫苗的祖先。
