根据一级和二级序列-结构分析，牛源经典猪瘟病毒株的基因组特征。
Genomic characteristics of classical swine fever virus strains of bovine origin according to primary and secondary sequence-structure analysis.
机构信息
Faculty of Veterinary Medicine, University of Teramo, Italy.
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, No 4899 Juye Street, Changchun, Jilin, People's Republic of China.
出版信息
Open Vet J. 2020 Apr;10(1):94-115. doi: 10.4314/ovj.v10i1.15. Epub 2020 Mar 27.
BACKGROUND
Classical swine fever virus (CSFV), species member of the family , is generally considered restricted to domestic and wild suids. The circulation of CFSV has been detected in cattle herds in China and India. Natural infection appeared associated with clinical signs in some cases.
AIM
The secondary structures of the internal ribosome entry site in the 5' untranslated region (UTR) were used for the genomic characterization of bovine strains.
METHODS
Sequences have been compared to the representative CSFV strains isolated from pigs, vaccines, and contaminants from porcine cell lines and an ovine strain isolated in Spain.
RESULTS
The observed sequences from cattle showed a genetic relatedness with live-attenuated vaccine strains used in pigs. Sequence characteristics of the Chinese strain S171 are genetically distant from the previously reported CSFV genotypes, suggesting a new outgroup in the species, described for the first time, and named CSFV-d. Other Chinese strains were genetically closely related to CSFV genotype a2 (Alfort type) pig strains. Indian strains, reported from the states of Tamil Nadu and Meghalaya, were genetically closely related to CSFV genotype a1 (Brescia type) and a5 pig strains, respectively.
CONCLUSION
These preliminary observations are new and relevant in countries, where CSFV control and eradication strategies are applied.
背景
经典猪瘟病毒（CSFV），属于家族成员，通常被认为仅限于国内和野生猪科动物。在中国和印度的牛群中已经检测到了 CFSV 的循环。在某些情况下，自然感染与临床症状有关。
目的
使用 5'非翻译区（UTR）内部核糖体进入位点的二级结构对牛株进行基因组特征分析。
方法
对序列进行了比较，与从猪、疫苗和猪细胞系污染物中分离的代表 CSFV 株以及在西班牙分离的绵羊株进行了比较。
结果
从牛中观察到的序列与在猪中使用的活疫苗株具有遗传相关性。中国株 S171 的序列特征与先前报道的 CSFV 基因型在遗传上有很大差异，这表明该物种中出现了一个新的外群，这是首次描述的，并命名为 CSFV-d。其他中国株与 CSFV 基因型 a2（阿尔福类型）猪株在遗传上密切相关。来自泰米尔纳德邦和梅加拉亚邦的印度株与 CSFV 基因型 a1（布雷西亚类型）和 a5 猪株在遗传上密切相关。
结论
这些初步观察结果在实施 CSFV 控制和根除策略的国家具有新的和重要的意义。
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