Gomes Ana P, Sinclair David A
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
Methods Mol Biol. 2015;1241:49-57. doi: 10.1007/978-1-4939-1875-1_5.
Metabolic flexibility is vital for the cells to adapt to different energetic situations, allowing the organisms to adapt to changing conditions and survive challenges. One of the most important regulators of the metabolic flexibility is PGC-1α activity. PGC-1α integrates numerous signals and regulates a variety of transcription factors and nuclear receptors that together regulate mitochondrial homeostasis and fatty acid oxidation. One of the major ways that PGC-1α activity is regulated is by changes in its acetylation status. Thus measuring the acetylation status of PGC-1α is an important indicator of the metabolic flexibility of the cells. In this chapter, we describe an approach to evaluate PGC-1α acetylation in primary mouse myotubes. The method is applicable to other cell types and tissues as well.
代谢灵活性对于细胞适应不同的能量状况至关重要，使生物体能够适应不断变化的条件并应对挑战。代谢灵活性最重要的调节因子之一是PGC-1α的活性。PGC-1α整合众多信号并调节多种转录因子和核受体，这些因子共同调节线粒体稳态和脂肪酸氧化。调节PGC-1α活性的主要方式之一是通过其乙酰化状态的变化。因此，测量PGC-1α的乙酰化状态是细胞代谢灵活性的重要指标。在本章中，我们描述了一种评估原代小鼠肌管中PGC-1α乙酰化的方法。该方法也适用于其他细胞类型和组织。
