Department of Genetics, Blavatnik Institute, Paul F. Glenn Center for Biology of Aging Research, Harvard Medical School, Boston, MA, USA.
Center for Neurosciences and Cell Biology of the University of Coimbra, Coimbra, Portugal.
Methods Mol Biol. 2021;2310:301-309. doi: 10.1007/978-1-0716-1433-4_17.
Metabolic flexibility is vital for organisms to respond to and survive changes in energy availability. A critical metabolic flexibility regulator is peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), which regulates various transcription factors and nuclear receptors that, in turn, regulate mitochondrial homeostasis and fatty acid oxidation. PGC-1α is itself regulated, with one of the significant modes of regulation being acetylation. Thus, measuring the acetylation status of PGC-1α is a critical indicator of cells' metabolic flexibility. In this chapter, we describe a method of evaluating PGC-1α acetylation in primary mouse myotubes. This method can also be used with other cell types and tissues.
代谢灵活性对于生物体应对和适应能量供应变化至关重要。过氧化物酶体增殖物激活受体γ共激活因子 1α（PGC-1α）是一种关键的代谢灵活性调节剂，它调节各种转录因子和核受体，这些转录因子和核受体反过来又调节线粒体稳态和脂肪酸氧化。PGC-1α 本身受到调节，其中一种重要的调节方式是乙酰化。因此，测量 PGC-1α 的乙酰化状态是细胞代谢灵活性的一个关键指标。在本章中，我们描述了一种评估原代小鼠肌管中 PGC-1α 乙酰化的方法。该方法也可用于其他细胞类型和组织。
