Anter Jaouad, Tasset Inmaculada, Demyda-Peyrás Sebastián, Ranchal Isidora, Moreno-Millán Miguel, Romero-Jimenez Magdalena, Muntané Jordi, Luque de Castro María Dolores, Muñoz-Serrano Andrés, Alonso-Moraga Ángeles
Department of Genetics, University of Córdoba, Campus Rabanales, 14071 Córdoba, Spain.
Department of Biochemistry and Molecular Biology, School of Medicine, University of Córdoba, 14071 Córdoba, Spain.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Sep 15;772:25-33. doi: 10.1016/j.mrgentox.2014.07.002. Epub 2014 Jul 25.
Olive oil is an integral ingredient of the "Mediterranean diet". The olive oil industry generates large quantities of a by-product called "alperujo" (AL) during the two-phase centrifugation system developed in the early nineties. AL could be a potent exploitable source of natural phenolic antioxidants. Our results showed that AL and its distinctive phenols hydroxytyrosol, tyrosol and verbascoside were not genotoxic in the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster and exerted antigenotoxic activity against DNA oxidative damage generated by hydrogen peroxide (H2O2). Alperujo and hydroxytyrosol also exhibited notable antiproliferative and caspase 3-dependent proapoptotic effects toward the human tumoral cell line HL60. AL can provide a cheap and efficient source of chemopreventive phenolic compounds with strong antioxidant properties, becoming a promising and potent therapeutic drug in the future.
橄榄油是“地中海饮食”的重要组成部分。在九十年代初开发的两相离心系统中，橄榄油产业会产生大量名为“alperujo”（AL）的副产品。AL可能是一种潜在的可开发利用的天然酚类抗氧化剂来源。我们的结果表明，AL及其独特的酚类物质羟基酪醇、酪醇和毛蕊花糖苷在黑腹果蝇的体细胞突变和重组试验（SMART）中没有遗传毒性，并且对过氧化氢（H2O2）产生的DNA氧化损伤具有抗基因毒性活性。Alperujo和羟基酪醇对人肿瘤细胞系HL60也表现出显著的抗增殖和半胱天冬酶3依赖性促凋亡作用。AL可以提供一种廉价且高效的具有强大抗氧化特性的化学预防酚类化合物来源，在未来成为一种有前景且有效的治疗药物。
