Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, "Dunărea de Jos" University of Galaţi, 47 Domneasca Street, 800008 Galaţi, Romania.
Faculty of Chemistry, Alexandru Ioan Cuza University, 11 Carol I Bd., 700506 Iasi, Romania.
Int J Mol Sci. 2022 Dec 11;23(24):15704. doi: 10.3390/ijms232415704.
In addition to their antioxidant and antimicrobial action in functional foods, beverages, and in some dermato-cosmetic products, olive phenolic compounds are also recognized for their role in the prevention of diabetes and inflammation, treatment of heart disease and, consequently, of the numerous chronic diseases mediated by the free radicals. In recent years, attention has increased, in particular, regarding one of the most important compound in extra virgin olive oil (EVOO) having glycosidic structure, namely verbocoside, due to the existence in the literature of numerous studies demonstrating its remarkable contribution to the prophylaxis and treatment of various disorders of the human body. The purpose of this study was the qualitative and quantitative determination of verbascoside in commercial EVOOs from different regions by means of a newly developed sensor based on a screen-printed carbon electrode (SPCE) modified with graphene oxide (GPHOX), on the surface of which a pentapeptide was immobilized by means of glutaraldehyde as cross-linking agent. The modified electrode surface was investigated using both Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. This newly developed sensor has shown a high sensibility compared to the unmodified electrode, a low detection limit (LOD) of up to 9.38 × 10 M, and a wide linearity range between 0.1 µM and 10.55 µM. The applicability of the modified sensor was confirmed by detecting verbascoside in ten different EVOOs samples using the cyclic voltammetry (CV) method, with very good results. The validation of the electroanalytical method was performed by using the standard addition method with very good recoveries in the range of 97.48-103.77%.
除了在功能性食品、饮料和某些皮肤化妆品产品中具有抗氧化和抗菌作用外，橄榄酚类化合物还因其在预防糖尿病和炎症、治疗心脏病以及因此预防自由基介导的许多慢性疾病方面的作用而受到认可。近年来，人们特别关注特级初榨橄榄油（EVOO）中一种具有糖苷结构的最重要化合物——毛蕊花糖苷，因为文献中存在大量研究证明其对预防和治疗人体各种疾病具有显著贡献。本研究的目的是通过一种基于氧化石墨烯（GPHOX）修饰的丝网印刷碳电极（SPCE）的新型传感器，对来自不同地区的商业 EVOO 中的毛蕊花糖苷进行定性和定量测定，在该传感器的表面上，通过戊二醛作为交联剂固定五肽。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）方法对修饰电极表面进行了研究。与未修饰电极相比，新开发的传感器具有更高的灵敏度、更低的检测限（LOD）可达 9.38×10 M，以及在 0.1 µM 和 10.55 µM 之间的宽线性范围。通过使用循环伏安法（CV）方法在十种不同的 EVOO 样品中检测毛蕊花糖苷，证明了修饰传感器的适用性，结果非常好。通过使用标准加入法对电分析方法进行验证，回收率在 97.48-103.77%之间非常好。
