用于……的纸质分子诊断
Paper-based molecular diagnostic for .
作者信息
Linnes Jacqueline C, Fan Andy, Rodriguez Natalia M, Lemieux Bertrand, Kong Huimin, Klapperich Catherine M
机构信息
Biomedical Engineering, Boston University, Boston, MA, 02215.
BioHelix Corporation, Beverly, MA, 01915.
出版信息
RSC Adv. 2014 Jan 1;4(80):42245-42251. doi: 10.1039/C4RA07911F.
Abstract
Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused by . This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only a pressure source and heat block, eliminating the need for expensive laboratory equipment. This paper-based test can be performed in less than one hour and has a clinically relevant limit of detection that is 100x more sensitive than current rapid immunoassays used for chlamydia diagnosis.
摘要
在此，我们展示了一种用于即时检测由……引起的性传播感染的最少仪器化纸质分子诊断方法的开发。这种新的诊断平台整合了细胞裂解、等温核酸扩增以及仅使用压力源和加热块的侧向流动视觉检测，无需昂贵的实验室设备。这种基于纸的检测可以在不到一小时内完成，并且具有临床相关的检测限，其灵敏度比目前用于衣原体诊断的快速免疫测定法高100倍。
相似文献
1
Paper-based molecular diagnostic for .用于……的纸质分子诊断
RSC Adv. 2014 Jan 1;4(80):42245-42251. doi: 10.1039/C4RA07911F.
2
Rapid and visual Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue.使用环介导等温扩增技术和羟基萘酚蓝快速可视化检测沙眼衣原体
Lett Appl Microbiol. 2017 Jan;64(1):51-56. doi: 10.1111/lam.12675. Epub 2016 Nov 21.
3
Combination with antimicrobial peptide lyses improves loop-mediated isothermal amplification based method for Chlamydia trachomatis detection directly in urine sample.与抗菌肽裂解相结合可改进基于环介导等温扩增的直接在尿液样本中检测沙眼衣原体的方法。
BMC Infect Dis. 2016 Jul 13;16:329. doi: 10.1186/s12879-016-1674-0.
4
Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections.用于性传播感染的即时护理分子诊断平台原型的模块化开发。
Med Eng Phys. 2016 Aug;38(8):741-8. doi: 10.1016/j.medengphy.2016.04.022. Epub 2016 May 26.
5
Progress and Perspectives in Point of Care Testing for Urogenital Chlamydia trachomatis Infection: A Review.即时检测在泌尿生殖道沙眼衣原体感染中的进展与展望：综述。
Med Sci Monit. 2020 Apr 16;26:e920873. doi: 10.12659/MSM.920873.
6
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae based on cross-priming amplification.基于交叉引物扩增法检测沙眼衣原体和淋病奈瑟菌
Lett Appl Microbiol. 2016 May;62(5):399-403. doi: 10.1111/lam.12560.
7
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA).使用基于多重链侵入扩增（mSIBA）技术检测沙眼衣原体和淋病奈瑟菌
Methods Mol Biol. 2019;2042:1-9. doi: 10.1007/978-1-4939-9694-0_1.
8
Diagnosis of Chlamydia infection in women.女性衣原体感染的诊断。
Taiwan J Obstet Gynecol. 2011 Sep;50(3):261-7. doi: 10.1016/j.tjog.2011.07.001.
9
Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae.环介导等温扩增法在沙眼衣原体和淋病奈瑟菌检测中的应用
Methods Mol Biol. 2019;2042:19-25. doi: 10.1007/978-1-4939-9694-0_3.
10
Paper-Based RNA Extraction, in Situ Isothermal Amplification, and Lateral Flow Detection for Low-Cost, Rapid Diagnosis of Influenza A (H1N1) from Clinical Specimens.基于纸的RNA提取、原位等温扩增及侧流检测用于从临床标本中低成本、快速诊断甲型H1N1流感
Anal Chem. 2015 Aug 4;87(15):7872-9. doi: 10.1021/acs.analchem.5b01594. Epub 2015 Jul 15.
引用本文的文献
1
Establishment of Sample-to-Answer Loop-Mediated Isothermal Amplification-Based Nucleic Acid Testing Using the Sampling, Processing, Incubation, Detection and Lateral Flow Immunoassay Platforms.基于采样、处理、孵育、检测和侧向流免疫分析平台建立基于样本到答案环介导等温扩增的核酸检测方法。
Biosensors (Basel). 2024 Dec 13;14(12):609. doi: 10.3390/bios14120609.
2
Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification.在巴氏吸管内使用新型非核酸纯化的等温扩增对血液疟原虫 RNA 进行即时检测。
Infect Dis Poverty. 2024 Oct 31;13(1):80. doi: 10.1186/s40249-024-01255-8.
3
Method for lysis and paper-based elution-free DNA extraction with colourimetric isothermal amplification.基于显色恒温扩增的裂解和无纸洗脱 DNA 提取方法。
Sci Rep. 2024 Jun 24;14(1):14479. doi: 10.1038/s41598-024-59763-4.
4
Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review.化学在核酸扩增检测（NAAT）样品制备中的趋势：综述。
Biosensors (Basel). 2023 Nov 10;13(11):980. doi: 10.3390/bios13110980.
5
A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms.一种用于从微生物中裂解和提取DNA和RNA的一次性化学加热器及干酶制剂。
Anal Methods. 2016 Apr 14;8(14):2880-2886. doi: 10.1039/c6ay00107f. Epub 2016 Mar 3.
6
LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.基于 LAMP 的即时检测生物传感器用于快速病原体检测。
Biosensors (Basel). 2022 Nov 23;12(12):1068. doi: 10.3390/bios12121068.
7
Rapid electrostatic DNA enrichment for sensitive detection of in clinical urinary samples.用于临床尿液样本中灵敏检测的快速静电DNA富集法。
Anal Methods. 2020 Feb 28;12(8):1085-1093. doi: 10.1039/c9ay02478f. Epub 2020 Feb 5.
8
Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.弥合即时检测核酸检测技术的发展与性传播感染患者护理之间的差距。
Lab Chip. 2022 Feb 1;22(3):476-511. doi: 10.1039/d1lc00665g.
9
Strategies for Engineering Affordable Technologies for Point-of-Care Diagnostics of Infectious Diseases.用于即时诊断传染病的经济实惠技术的工程策略。
Acc Chem Res. 2021 Oct 19;54(20):3772-3779. doi: 10.1021/acs.accounts.1c00434. Epub 2021 Oct 6.
10
Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification.采用 RNA 提取和等温扩增技术进行即时护理点 SARS-CoV-2 分子检测。
PLoS One. 2021 Jan 11;16(1):e0243712. doi: 10.1371/journal.pone.0243712. eCollection 2021.
本文引用的文献
1
Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation.快速微流控热裂解法用于诊断样品制备的性能评估。
Diagnostics (Basel). 2013 Jan 17;3(1):105-16. doi: 10.3390/diagnostics3010105.
2
Abortion Surveillance - United States, 2012.人工流产监测-美国，2012 年。
MMWR Surveill Summ. 2015 Nov 27;64(10):1-40. doi: 10.15585/ss6410a1.
3
A Portable, Pressure Driven, Room Temperature Nucleic Acid Extraction and Storage System for Point of Care Molecular Diagnostics.一种用于即时分子诊断的便携式、压力驱动、室温核酸提取与存储系统。
Anal Methods. 2013 Jul 7;5(13):3177-3184. doi: 10.1039/C3AY40162F.
4
Low cost extraction and isothermal amplification of DNA for infectious diarrhea diagnosis.低成本提取和等温扩增 DNA 用于感染性腹泻诊断。
PLoS One. 2013;8(3):e60059. doi: 10.1371/journal.pone.0060059. Epub 2013 Mar 28.
5
Purification of DNA/RNA in a microfluidic device.在微流控装置中纯化DNA/RNA。
Methods Mol Biol. 2013;949:403-11. doi: 10.1007/978-1-62703-134-9_25.
6
A lateral flow assay for quantitative detection of amplified HIV-1 RNA.用于定量检测扩增的 HIV-1 RNA 的侧向流动检测。
PLoS One. 2012;7(9):e45611. doi: 10.1371/journal.pone.0045611. Epub 2012 Sep 21.
7
Perceptions on Point-of-Care Tests for Sexually Transmitted Infections - Comparison between Frontline Clinicians and Professionals in Industry.关于性传播感染即时检测的认知——一线临床医生与行业专业人士的比较
Point Care. 2012 Jun 1;11(2):126-129. doi: 10.1097/POC.0b013e31825a25e8.
8
A paper and plastic device for performing recombinase polymerase amplification of HIV DNA.一种用于进行 HIV DNA 重组酶聚合酶扩增的纸质和塑料设备。
Lab Chip. 2012 Sep 7;12(17):3082-8. doi: 10.1039/c2lc40423k. Epub 2012 Jun 26.
9
Can mailed swab samples be dry-shipped for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis by nucleic acid amplification tests?邮寄拭子样本能否通过核酸扩增试验（NAAt）用于沙眼衣原体、淋病奈瑟菌和阴道毛滴虫的检测？
Diagn Microbiol Infect Dis. 2012 May;73(1):16-20. doi: 10.1016/j.diagmicrobio.2012.02.008.
10
A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami.一种低成本即时粘性样本制备装置，用于发展中国家的分子诊断；微流控折纸术的一个实例。
Lab Chip. 2012 Jan 7;12(1):174-81. doi: 10.1039/c1lc20622b. Epub 2011 Nov 8.
Zotero 插件