Retamales Hernan A, Scharaschkin Tanya
School of Earth, Environmental, and Biological Sciences, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4001 Australia.
Appl Plant Sci. 2014 Oct 3;2(10). doi: 10.3732/apps.1400063. eCollection 2014 Oct.
Here we propose a staining protocol using toluidine blue (TBO) and ruthenium red to reliably identify secondary compounds in the leaves of some species of Myrtaceae. •
Leaves of 10 species representing 10 different genera of Myrtaceae were processed and stained using five different combinations of ruthenium red and TBO. Optimal staining conditions were determined as 1 min of ruthenium red (0.05% aqueous) and 45 s of TBO (0.1% aqueous). Secondary compounds clearly identified under this treatment include mucilage in the mesophyll, polyphenols in the cuticle, lignin in fibers and xylem, tannins and carboxylated polysaccharides in the epidermis, and pectic substances in the primary cell walls. •
Potential applications of this protocol include systematic, phytochemical, and ecological investigations in Myrtaceae. It might be applicable to other plant families rich in secondary compounds and could be used as a preliminary screening method for extraction of these elements.
在此，我们提出一种使用甲苯胺蓝（TBO）和钌红的染色方案，以可靠地鉴定桃金娘科某些物种叶片中的次生化合物。
对代表桃金娘科10个不同属的10个物种的叶片进行处理，并使用钌红和TBO的五种不同组合进行染色。确定最佳染色条件为钌红（0.05%水溶液）染色1分钟，TBO（0.1%水溶液）染色45秒。在此处理下清晰鉴定出的次生化合物包括叶肉中的黏液、角质层中的多酚、纤维和木质部中的木质素、表皮中的单宁和羧化多糖，以及初生细胞壁中的果胶物质。
该方案的潜在应用包括在桃金娘科进行系统、植物化学和生态学研究。它可能适用于其他富含次生化合物的植物科，并可作为提取这些成分的初步筛选方法。
