Sonoda Shozo, Sakamoto Taiji, Shirasawa Makoto, Yamashita Takehiro, Uchino Eisuke, Terasaki Hiroto
Department of Ophthalmology, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Curr Eye Res. 2014 Dec;39(12):1200-6. doi: 10.3109/02713683.2014.888454. Epub 2014 Oct 13.
BACKGROUND/PURPOSE: To see the relationship between blood components and optical coherence tomography (OCT) reflectivity using an animal model in which the aqueous humor was substituted by different experimental solutions without changing the integrity of the retina.
The aqueous humor of an enucleated swine eye was replaced with plasma obtained from healthy volunteers. The OCT reflectivity of the anterior chamber filled with each plasma was calculated from individual OCT images, and was expressed by an arbitrary unit (AU). The concentration of blood components such as cholesterol, hemoglobin (Hb) and bilirubin of each individual was measured, and the correlation between each of them and the OCT reflectivity of aqueous humor in an enucleated swine eye was analyzed. Using the same model, the effects of the single plasma component on OCT reflectivity were examined.
Blood samples were obtained from 24 individuals. OCT reflectivity was 30.68 ± 14.8 AU (average ± SD), ranging from 11.11 to 60.31 AU. OCT reflectivity correlated significantly with the concentration of triglycerides (R = 0.634, p = 0.001) and total cholesterol (R = 0.488, p = 0.015) using Spearman's rank correlation coefficient. While a partial correlation analysis showed that it correlated significantly with triglyceride (R = 0.60, p = 0.003), but not total cholesterol. OCT reflectivity was highest in a balanced salt solution (BSS) with Hb (average 42.05 AU), followed by fibrinogen (8.08 AU), bilirubin (6.12 AU) and γ-globulin (2.85 AU). Albumin did not increase the reflectivity of the BSS with a normal concentration (1.11 AU) compared to the control BSS alone (0.73 AU).
OCT reflectivity was most strongly affected by the presence of triglycerides among the blood components. Some molecules such as Hb and fibrinogen significantly increase the OCT reflectivity. This information should be helpful for interpreting the OCT findings correctly.
背景/目的：利用动物模型观察血液成分与光学相干断层扫描（OCT）反射率之间的关系，该模型中房水被不同的实验溶液替代，而视网膜的完整性未改变。
将摘除眼球的猪眼中的房水用从健康志愿者获得的血浆替代。从各个OCT图像计算充满每种血浆的前房的OCT反射率，并用任意单位（AU）表示。测量每个个体的血液成分如胆固醇、血红蛋白（Hb）和胆红素的浓度，并分析它们与摘除眼球的猪眼中房水的OCT反射率之间的相关性。使用相同模型，研究单一血浆成分对OCT反射率的影响。
从24名个体采集血液样本。OCT反射率为30.68±14.8 AU（平均值±标准差），范围为11.11至60.31 AU。使用Spearman等级相关系数，OCT反射率与甘油三酯浓度（R = 0.634，p = 0.001）和总胆固醇浓度（R = 0.488，p = 0.015）显著相关。而偏相关分析表明它与甘油三酯显著相关（R = 0.60，p = 0.003），但与总胆固醇无关。在含有Hb的平衡盐溶液（BSS）中OCT反射率最高（平均42.05 AU），其次是纤维蛋白原（8.08 AU）、胆红素（6.12 AU）和γ-球蛋白（2.85 AU）。与单独的对照BSS（0.73 AU）相比，正常浓度的白蛋白不会增加BSS的反射率（1.11 AU）。
在血液成分中，OCT反射率受甘油三酯存在的影响最大。一些分子如Hb和纤维蛋白原会显著增加OCT反射率。这些信息有助于正确解释OCT检查结果。
