Kuo C H, Watanabe Y, Yamagata K, Miki N
Department of Pharmacology, Kanazawa University, Japan.
Brain Res Dev Brain Res. 1989 Nov 1;50(1):139-41. doi: 10.1016/0165-3806(89)90133-8.
Antisera raised against photoreceptor-specific MEKA and opsin proteins were provided for immunohistochemical studies on the retinas of normal (BJ57BL/6) and retinal degeneration (rd, C3H/He) mice. The expression of MEKA protein began at postnatal day 6 (P6) in the photoreceptor cells and gradually increased until P10 in both normal and rd mice. Thereafter the MEKA proteins in the photoreceptor cells of rd mice gradually decreased and disappeared at P18, whereas those of normal mice were increasing until P18. The time course of MEKA proteins in the photoreceptor cells of normal and rd mice was almost similar to that of opsin protein, except that the MEKA protein disappeared earlier than opsin.
制备了针对光感受器特异性MEKA和视蛋白的抗血清，用于对正常（BJ57BL/6）和视网膜变性（rd，C3H/He）小鼠视网膜进行免疫组织化学研究。在正常和rd小鼠中，MEKA蛋白的表达在出生后第6天（P6）开始于光感受器细胞，并逐渐增加，直到P10。此后，rd小鼠光感受器细胞中的MEKA蛋白逐渐减少，并在P18消失，而正常小鼠的MEKA蛋白在P18之前一直在增加。正常和rd小鼠光感受器细胞中MEKA蛋白的时间进程与视蛋白几乎相似，只是MEKA蛋白比视蛋白消失得更早。
