Jo Yun Kee, Seo Jeong Hyun, Choi Bong-Hyuk, Kim Bum Jin, Shin Hwa Hui, Hwang Byeong Hee, Cha Hyung Joon
Department of Chemical Engineering, Pohang University of Science and Technology , Pohang 790-784, Korea.
ACS Appl Mater Interfaces. 2014 Nov 26;6(22):20242-53. doi: 10.1021/am505784k. Epub 2014 Oct 23.
During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.
在植入手术过程中，需要使用抗菌剂来预防细菌感染，细菌感染会导致植入材料与组织之间形成生物膜。源自海洋贻贝的贻贝黏附蛋白（MAPs）是一种生物黏合剂，即使在潮湿环境中也能在各种表面上表现出强大的黏附力和包覆能力。在此，我们提出了一种基于将MAP与银结合肽融合的新型非依赖表面的抗菌涂层策略，该策略可合成具有广泛抗菌活性的银纳米颗粒。这种粘性重组融合蛋白能够在目标表面上高效包覆，并在温和条件下在包覆表面上轻松生成银纳米颗粒。生物合成的银纳米颗粒对革兰氏阳性菌和革兰氏阴性菌均表现出优异的抗菌效果，并且与哺乳动物细胞也具有良好的细胞相容性。在这种涂层策略中，MAP-银结合肽融合蛋白提供了包含无机银纳米颗粒的混合环境，同时介导了银纳米颗粒与周围环境的相互作用。此外，银纳米颗粒通过一种简单的、非依赖表面的包覆方式在包括金属、塑料和玻璃在内的各种表面上完全合成，并且它们也成功地在通过融合蛋白静电纺丝制备的纳米纤维表面上合成。因此，这种简便的非依赖表面的银纳米颗粒生成抗菌涂层在预防各种生物医学领域的细菌感染方面具有巨大的应用潜力。
