Department of Molecular Biosciences.
Department of Molecular Biosciences,
J Gerontol A Biol Sci Med Sci. 2015 Oct;70(10):1181-8. doi: 10.1093/gerona/glu177. Epub 2014 Oct 13.
Calorie restriction (CR) without malnutrition extends life span in several animal models. It has been proposed that a decrease in the amount of polyunsaturated fatty acids (PUFAs), and especially n-3 fatty acids, in membrane phospholipids may contribute to life span extension with CR. Phospholipid PUFAs are sensitive to dietary fatty acid composition, and thus, the purpose of this study was to determine the influence of dietary lipids on life span in CR mice. C57BL/6J mice were assigned to four groups (a 5% CR control group and three 40% CR groups) and fed diets with soybean oil (high in n-6 PUFAs), fish oil (high in n-3 PUFAs), or lard (high in saturated and monounsaturated fatty acids) as the primary lipid source. Life span was increased (p < .05) in all CR groups compared to the Control mice. Life span was also increased (p < .05) in the CR lard mice compared to animals consuming either the CR fish or soybean oil diets. These results indicate that dietary lipid composition can influence life span in mice on CR, and suggest that a diet containing a low proportion of PUFAs and high proportion of monounsaturated and saturated fats may maximize life span in animals maintained on CR.
热量限制（CR）在没有营养不良的情况下可以延长几种动物模型的寿命。有人提出，细胞膜磷脂中多不饱和脂肪酸（PUFAs），特别是 n-3 脂肪酸含量的减少可能有助于 CR 延长寿命。磷脂 PUFAs 对饮食脂肪酸组成敏感，因此，本研究旨在确定饮食脂质对 CR 小鼠寿命的影响。C57BL/6J 小鼠被分为四组（5%CR 对照组和 3 个 40%CR 组），并喂食以大豆油（富含 n-6 PUFAs）、鱼油（富含 n-3 PUFAs）或猪油（富含饱和和单不饱和脂肪酸）为主要脂质来源的饮食。与对照组相比，所有 CR 组的寿命都有所延长（p<.05）。与食用 CR 鱼油或大豆油饮食的动物相比，CR 猪油组的寿命也有所延长（p<.05）。这些结果表明，饮食脂质组成可以影响 CR 小鼠的寿命，并表明含有低比例 PUFAs 和高比例单不饱和和饱和脂肪的饮食可能最大限度地延长 CR 维持动物的寿命。
