Li Yan, Shan Yunfeng, Chi Ying, Wen Tian, Han Xiaodong
Department of Histology, Medical School, Nanjing University, Nanjing, Jiangsu 210093, China.
Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, China.
Chin Med J (Engl). 2014;127(20):3576-80.
H9N2 avian influenza viruses (AIVs) have repeatedly caused infections in mammals even humans in many countries. The purpose of our study was to evaluate the acute lung injury (ALI) caused by H9N2 viral infection in mice.
Six- to eight- week-old female SPF C57BL/6 mice were infected intranasally with 1 × 10(4) MID50 of A/HONG KONG/2108/2003 [H9N2 (HK)] virus. Clinical signs, pathological changes, virus titration in tissues of mice, arterial blood gas, and cytokines in bronchoalveolar lavage fluid (BALF) and serum were observed at different time points after AIV infection.
H9N2-AIV-infected mice exhibited severe respiratory syndrome, with a mortality rate of 50%. Lung histopathological changes in infected mice included diffuse pneumonia, alveolar damage, inflammatory cellular infiltration, interstitial and alveolar edema, and hemorrhage. In addition, H9N2 viral infection resulted in severe progressive hypoxemia, lymphopenia, and a significant increase in interleukin 1, interleukin 6, tumor necrosis factor, and interferon in BALF and serum.
The results suggest that H9N2 viral infection induces a typical ALI in mice that resembles the common features of ALI. Our data may facilitate the future studies of potential avian H9N2 disease in humans.
H9N2禽流感病毒（AIVs）在许多国家多次导致哺乳动物甚至人类感染。我们研究的目的是评估H9N2病毒感染小鼠所引起的急性肺损伤（ALI）。
6至8周龄的雌性SPF C57BL/6小鼠经鼻内感染1×10⁴半数感染剂量（MID50）的A/香港/2108/2003 [H9N2（HK）]病毒。在禽流感病毒感染后的不同时间点观察小鼠的临床症状、病理变化、组织中的病毒滴定、动脉血气以及支气管肺泡灌洗液（BALF）和血清中的细胞因子。
感染H9N2-AIV的小鼠表现出严重的呼吸综合征，死亡率为50%。感染小鼠的肺组织病理学变化包括弥漫性肺炎、肺泡损伤、炎性细胞浸润、间质和肺泡水肿以及出血。此外，H9N2病毒感染导致严重的进行性低氧血症、淋巴细胞减少，并且BALF和血清中的白细胞介素1、白细胞介素6、肿瘤坏死因子和干扰素显著增加。
结果表明，H9N2病毒感染在小鼠中诱发了典型的ALI，类似于ALI的常见特征。我们的数据可能有助于未来对人类潜在禽H9N2疾病的研究。
