烟曲霉脉冲树突状细胞在体外诱导的Th和调节性T细胞反应
Th and Treg response induced by Aspergillus fumigatus pulsed dendritic cells in vitro.
作者信息
Wang Runchao, Wan Zhe, Li Ruoyu
机构信息
Department of Dermatology, Research Center for Medical Mycology, Peking University First Hospital, Peking University, Beijing 100034, China.
Department of Dermatology, Research Center for Medical Mycology, Peking University First Hospital, Peking University, Beijing 100034, China. Email:
出版信息
Chin Med J (Engl). 2014;127(20):3616-22.
BACKGROUND
Dendritic cells (DCs) can recognize the pathogen-associated molecular patterns (PAMP) of Aspergillus fumigatus (A. fumigatus), activating the immune response. During A. fumigatus infection, a Th and Treg response induced in the fungi-pulsed DCs is not yet well understood.
METHODS
In this study, bone marrow-derived dendritic cells (BMDCs) were separated and proliferated from C57BL/6 mice. A. fumigatus pulsed DCs were generated and cultured with CD4(+) T cells derived from the spleen of C57BL/6 mice in vitro. CD4(+) T cells differentiation after co-culture were analyzed by flow cytometry, ELISA, and real-time PCR analysis.
RESULTS
The A. fumigatus pulsed DCs exhibited increased Th1 and Treg frequency, Th1-related cytokines (IFN-γ and IL-12), Treg-related cytokines (TGF-β) and T-bet, and Foxp3 mRNA levels compared with the control group. There was no significant difference between A. fumigatus pulsed DCs group and the control group about Th17 and Th2 frequency.
CONCLUSIONS
The inactivated conidia of A. fumigatus were able to activate BMDCs and made them capable of triggering T cell responses in vitro. A. fumigatus loaded DCs was a weak inducer of Th17 and Th2, but induced a strong Th1 and Treg response.
背景
树突状细胞（DCs）能够识别烟曲霉（A. fumigatus）的病原体相关分子模式（PAMP），从而激活免疫反应。在烟曲霉感染期间，真菌刺激的DCs中诱导的Th和Treg反应尚未完全清楚。
方法
在本研究中，从C57BL/6小鼠中分离并增殖骨髓来源的树突状细胞（BMDCs）。体外培养烟曲霉刺激的DCs，并与来自C57BL/6小鼠脾脏的CD4(+) T细胞共培养。通过流式细胞术、酶联免疫吸附测定（ELISA）和实时聚合酶链反应（PCR）分析共培养后CD4(+) T细胞的分化情况。
结果
与对照组相比，烟曲霉刺激的DCs表现出Th1和Treg频率增加，Th1相关细胞因子（IFN-γ和IL-12）、Treg相关细胞因子（TGF-β）以及T-bet和Foxp3 mRNA水平升高。烟曲霉刺激的DCs组与对照组在Th17和Th2频率方面无显著差异。
结论
烟曲霉的灭活分生孢子能够激活BMDCs，并使其能够在体外触发T细胞反应。负载烟曲霉的DCs是Th17和Th2的弱诱导剂，但可诱导强烈的Th1和Treg反应。
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