Farrokhi Effat, Samani Keihan Ghatreh, Chaleshtori Morteza Hashemzadeh
Cellular and Molecular Research Center and.
Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Lab Med. 2014 Fall;45(4):297-301. doi: 10.1309/LMUJWVQFW6CJMSOQ.
Oxidative stress has been associated with the progression of atherosclerosis and activation of genes that lead to increased deposition of proteins in the extracellular matrix. Bone sialoprotein (BSP) and osteonectin are proteins involved in the initiation and progression of vascular calcification.
To investigate the effect of oxidized low-density lipoprotein on osteonectin and BSP expression in human aorta vascular smooth muscle cells (HA/VSMCs).
We treated HA/VSMCs with oxidized low-density lipoprotein (oxLDL) and measured the relative expression of osteonectin and BSP genes using the real-time polymerase chain reaction (PCR) method. We investigated the protein levels produced by each gene using the western blotting technique.
oxLDL increased osteonectin and BSP levels (mean [SD], 9.1 [2.1]-fold and 4.2 [0.75]-fold, respectively) after 48 hours. The western blotting results also confirmed the increased levels of osteonectin and BSP.
oxLDL may enhance vascular calcification by promoting the expression of osteonectin and BSP.
氧化应激与动脉粥样硬化的进展以及导致细胞外基质中蛋白质沉积增加的基因激活有关。骨唾液蛋白（BSP）和骨连接蛋白是参与血管钙化起始和进展的蛋白质。
研究氧化型低密度脂蛋白对人主动脉血管平滑肌细胞（HA/VSMCs）中骨连接蛋白和BSP表达的影响。
用氧化型低密度脂蛋白（oxLDL）处理HA/VSMCs，并使用实时聚合酶链反应（PCR）方法测量骨连接蛋白和BSP基因的相对表达。我们使用蛋白质印迹技术研究每个基因产生的蛋白质水平。
48小时后，oxLDL使骨连接蛋白和BSP水平升高（平均值[标准差]分别为9.1[2.1]倍和4.2[0.75]倍）。蛋白质印迹结果也证实了骨连接蛋白和BSP水平的升高。
oxLDL可能通过促进骨连接蛋白和BSP的表达来增强血管钙化。
