Niknam Sara, Ghatreh-Samani Keihan, Farrokhi Effat
1.Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2.Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Int J Mol Cell Med. 2015 Winter;4(1):60-6.
Osteonectin is a bone- associated protein involved in vascular calcification. Adiponectin may protect against cardiovascular disease but possible effects on vascular calcification have been poorly studied. The aim of this study was to investigate the modulatory effect of adiponectin on oxidized low density lipoprotein (oxLDL)- induced expression of osteonectin in human aorta vascular smooth muscle cells (HA/VSMCs). HA/VSMCs were cultured in F12K media and then treated with oxLDL (100 µg/mL) in the presence or absence of adoponectin (5 µg/mL) for 24 and 48 hours. mRNA expression and protein level of osteonectin were determined by quantitative real-time PCR and western blot analysis, respectively. After exposure to oxLDL, osteonectin expression increased 1.62 ± 0.23- and 6.62 ± 0.48-fold after 24 and 48 hours respectively compared to the control. Adiponectin increased oxLDL- induced osteonectin expression in a time-dependent manner after 24 and 48 hours (3.24 ± 0.39- and 24.93 ± 2.15-fold, respectively). Western blotting confirmed that osteonectin protein was upregulated by adiponectin.Our data suggest that OxLDL might cause the increase of osteonectin expression both at mRNA and protein level. This upregulation is intensified by adiponectin.
骨连接蛋白是一种与骨相关的蛋白质，参与血管钙化过程。脂联素可能对心血管疾病具有保护作用，但对血管钙化的潜在影响研究较少。本研究旨在探讨脂联素对氧化型低密度脂蛋白（oxLDL）诱导的人主动脉血管平滑肌细胞（HA/VSMCs）中骨连接蛋白表达的调节作用。将HA/VSMCs培养于F12K培养基中，然后在存在或不存在脂联素（5μg/mL）的情况下用oxLDL（100μg/mL）处理24小时和48小时。分别通过定量实时PCR和蛋白质印迹分析测定骨连接蛋白的mRNA表达和蛋白水平。与对照组相比，暴露于oxLDL后，24小时和48小时后骨连接蛋白表达分别增加了1.62±0.23倍和6.62±0.48倍。脂联素在24小时和48小时后以时间依赖性方式增加oxLDL诱导的骨连接蛋白表达（分别为3.24±0.39倍和24.93±2.15倍）。蛋白质印迹证实脂联素上调了骨连接蛋白的蛋白水平。我们的数据表明，oxLDL可能在mRNA和蛋白质水平上导致骨连接蛋白表达增加。脂联素会加剧这种上调作用。
