基于质谱的维生素D检测中手动与自动样本处理的工作流程分析比较
Workflow analysis comparing manual and automated specimen processing for mass spectrometry-based vitamin D testing.
作者信息
Tacker Danyel Hermes, Topardo Joyce, Mahaffey Carole, Perrotta Peter L
机构信息
Clinical Laboratory, Department of Pathology, West Virginia University Hospital, Morgantown, West Virginia
Clinical Laboratory, Department of Pathology, West Virginia University Hospital, Morgantown, West Virginia.
出版信息
Lab Med. 2014 Fall;45(4):361-7. doi: 10.1309/LMZL47EN6KDODMXJ.
OBJECTIVE
To quantify the benefits of automating specimen extraction in terms of specimen-preparation times and labor usage.
METHODS
We used workflow modeling and time-motion studies to compare manual and automated solid-phase extraction methods to prepare specimens for a mass spectrometry-based vitamin D assay. We processed 20 batches, that included 5 to 90 specimens each, with both methods in parallel and randomly over a 4-week period. Technologist discomfort/fatigue was subjectively measured.
RESULTS
Batch preparation time, per-specimen processing time, and labor requirements were significantly lower for all batch sizes on the Tecan Freedom EVO 150 robotic liquid-handling system (EVO). Technologist fatigue was significant when batch sizes reached 60 specimens. Cycle times were more uniform on the EVO. Automation provided as many as 85 minutes of useable technologist idle time for the 90-specimen batch.
CONCLUSIONS
Automated specimen preparation should be considered when batch sizes reach 35 to 40 specimens per day.
目的
从样本制备时间和劳动力使用方面量化自动化样本提取的益处。
方法
我们使用工作流程建模和时间动作研究，比较手动和自动固相萃取方法，为基于质谱的维生素D检测制备样本。在4周时间内，我们同时并行且随机地用这两种方法处理了20批样本，每批包含5至90个样本。对技术人员的不适/疲劳进行主观测量。
结果
在Tecan Freedom EVO 150机器人液体处理系统（EVO）上，所有批次大小的批次制备时间、每个样本的处理时间和劳动力需求均显著更低。当批次大小达到60个样本时，技术人员疲劳显著。EVO上的循环时间更均匀。对于90个样本的批次，自动化为技术人员提供了多达85分钟的可用空闲时间。
结论
当每天的批次大小达到35至40个样本时，应考虑自动化样本制备。
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