Hahn Steven
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109.
Genetics. 2014 Oct;198(2):455-60. doi: 10.1534/genetics.114.167361.
Based on his work with the Escherichia coli l-arabinose operon, Ellis Englesberg proposed in 1965 that the regulatory gene araC was an "activator gene" required for positive control of the ara operon. This challenged the widely held belief in a universal mechanism of negative regulation proposed earlier by Jacob and Monod. For years, Englesberg's model was met with deep skepticism. Despite much frustration with complex ad hoc explanations used to challenge his model, Englesberg persisted until the evidence for positive control in ara and other systems became overwhelming. Englesberg's pioneering work enriched the original operon model and had a lasting impact in opening new and exciting ways of thinking about transcriptional regulation.
基于他对大肠杆菌L-阿拉伯糖操纵子的研究，埃利斯·恩格尔斯伯格在1965年提出，调节基因araC是阿拉伯糖操纵子正向调控所需的“激活基因”。这挑战了雅各布和莫诺德早期提出的普遍存在的负调控机制这一广泛信念。多年来，恩格尔斯伯格的模型遭到了深深的怀疑。尽管对用于质疑其模型的复杂临时解释感到非常沮丧，但恩格尔斯伯格坚持了下来，直到阿拉伯糖及其他系统中正向调控的证据变得压倒性。恩格尔斯伯格的开创性工作丰富了原始的操纵子模型，并对开启关于转录调控的全新且令人兴奋的思维方式产生了持久影响。
