Nacopoulos Cleopatra, Dontas Ismene, Lelovas Pavlos, Galanos Antonis, Vesalas Anna-Maria, Raptou Panagiota, Mastoris Michael, Chronopoulos Eustathios, Papaioannou Nikolaos
From the *Laboratory for Research of the Musculoskeletal System, School of Medicine, University of Athens; and †Biomedical Research Foundation of the Academy of Athens, Athens, Greece; ‡School of Dentistry, Comenius University, Bratislava, Slovakia; and § "Digital Dental Imaging" and ∥Department of Orthopedic Surgery, School of Medicine, University of Athens, Athens, Greece.
J Craniofac Surg. 2014 Nov;25(6):2164-8. doi: 10.1097/SCS.0000000000001172.
Platelet-rich fibrin (PRF) is a relatively new developed platelet concentrate with several benefits over platelet-rich plasma. The aim of this study was to compare healing properties of PRF and its combination with a ceramic synthetic material (graft) composed of hydroxyapatite and b-tricalcium phosphate in an animal model.
A bone deficit was surgically created in each femoral condyle of 15 New Zealand white rabbits. In each animal, 1 limb had (a) PRF only and the other (b) PRF plus synthetic graft material randomly implanted. Experimental animals were killed 3 months postoperatively. Histological and radiological examinations were made by means of computed tomography and peripheral quantitative computed tomography.
Mean density of the healed bone was statistically significantly greater when synthetic material was used (P < 0.0005). Moreover, combination of PRF with the synthetic material resulted in more cortical and subcortical bone formation (P = 0.038 and P = 0.037, respectively).
The addition of the ceramic material significantly increased the formation of new bone, providing a better substrate for bone regeneration.
富血小板纤维蛋白（PRF）是一种新开发的血小板浓缩物，相较于富血小板血浆有诸多优势。本研究旨在比较PRF及其与由羟基磷灰石和β-磷酸三钙组成的陶瓷合成材料（移植物）组合在动物模型中的愈合特性。
在15只新西兰白兔的每个股骨髁上手术制造骨缺损。在每只动物中，1条肢体仅植入（a）PRF，另1条肢体随机植入（b）PRF加合成移植物材料。实验动物在术后3个月处死。通过计算机断层扫描和外周定量计算机断层扫描进行组织学和放射学检查。
使用合成材料时，愈合骨的平均密度在统计学上显著更高（P < 0.0005）。此外，PRF与合成材料的组合导致更多的皮质骨和皮质下骨形成（分别为P = 0.038和P = 0.037）。
添加陶瓷材料显著增加了新骨形成，为骨再生提供了更好的基质。
