Lee Eui-Hee, Kim Jwa-Young, Kweon Hae Yong, Jo You-Young, Min Soo-Kee, Park Young-Wook, Choi Je-Yong, Kim Seong-Gon
Department of Oral and Maxillofacial Surgery, Sacred Heart Hospital, Hallym University, Gangneung, Korea.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 May;109(5):e33-8. doi: 10.1016/j.tripleo.2009.12.043. Epub 2010 Feb 10.
The objective of this study was to determine the capabilities of silk fibroin as a biomaterial template for bone formation when mixed with Choukroun platelet-rich fibrin (PRF) in vivo.
Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter 9.0 mm). The silk fibroin was digested by acid and made into powder (molecular weight <1.0 kDa). The right side (experimental group) received the silk fibroin plus platelet-rich fibroin and the left side (control group) did not receive a graft. Animals were killed at 6 weeks and 12 weeks. The specimens were examined by microscopic computerized tomography (micro-CT). Subsequently, they underwent decalcification and were stained for histologic analysis.
There was no significant difference between groups at 6 weeks after operation. In the micro-CT results, however, tissue mineral content in the experimental group at 12 weeks after operation was 132.09 +/- 4.41 and that in the control group was 126.42 +/- 6.62 (P = .011). Tissue mineral density in the experimental group was 2,088.88 +/- 648.34, and that in the control group was 2,029.72 +/- 668.22 (P = .013). The results of the histomorphometric analysis were in accordance with the micro-CT results. The total new bone was 49.86 +/- 7.49% in the control group at 12 weeks after the operation and 59.83 +/- 10.92% in the experimental group (P = .021).
A combined application of Choukroun PRF with acid-digested silk fibroin showed more rapid bone healing than unfilled control.
本研究的目的是确定丝素蛋白与乔克鲁恩富血小板纤维蛋白（PRF）在体内混合时作为骨形成生物材料模板的能力。
本研究使用了10只新西兰白兔，在顶骨上形成双侧圆形缺损（直径9.0毫米）。丝素蛋白经酸消化制成粉末（分子量<1.0 kDa）。右侧（实验组）接受丝素蛋白加富血小板纤维蛋白，左侧（对照组）不接受移植。在6周和12周时处死动物。通过显微计算机断层扫描（micro-CT）检查标本。随后，对其进行脱钙并进行组织学分析染色。
术后6周时两组之间无显著差异。然而，在micro-CT结果中，术后12周实验组的组织矿物质含量为132.09±4.41，对照组为126.42±6.62（P = 0.011）。实验组的组织矿物质密度为2,088.88±648.34，对照组为2,029.72±668.22（P = 0.013）。组织形态计量分析结果与micro-CT结果一致。术后12周时，对照组的新骨总量为49.86±7.49%，实验组为59.83±10.92%（P = 0.021）。
乔克鲁恩PRF与酸消化丝素蛋白联合应用比未填充对照组显示出更快的骨愈合。
