Yoon Jong-Suk, Lee Sang-Hwa, Yoon Hyun-Joong
Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea.
Department of Oral and Maxillofacial Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea, Seoul, Republic of Korea.
J Craniomaxillofac Surg. 2014 Oct;42(7):1071-7. doi: 10.1016/j.jcms.2014.01.034. Epub 2014 Jan 15.
The purpose of this study was to investigate the influence of platelet-rich fibrin (PRF) on angiogenesis and osteogenesis in guided bone regeneration (GBR) using xenogenic bone in rabbit cranial defects.
In each rabbit, 2 circular bone defects, one on either side of the midline, were prepared using a reamer drill. Each of the experimental sites received bovine bone with PRF, and each of the control sites received bovine bone alone. The animals were sacrificed at 1 week (n = 4), 2 weeks (n = 3) and 4 weeks (n = 3). Biopsy samples were examined histomorphometrically by light microscopy, and expression of vascular endothelial growth factor (VEGF) was determined by immunohistochemical staining.
At all experimental time points, immunostaining intensity for VEGF was consistently higher in the experimental group than in the control group. However, the differences between the control group and the experimental group were not statistically significant in the histomorphometrical and immunohistochemical examinations.
The results of this study suggest that PRF may increase the number of marrow cells. However, PRF along with xenogenic bone substitutes does not show a significant effect on bony regeneration. Further large-scale studies are needed to confirm our results.
本研究旨在探讨富血小板纤维蛋白（PRF）对兔颅骨缺损采用异种骨引导骨再生（GBR）中血管生成和成骨的影响。
在每只兔子中，使用扩孔钻在中线两侧制备2个圆形骨缺损。每个实验部位植入含PRF的牛骨，每个对照部位仅植入牛骨。在1周（n = 4）、2周（n = 3）和4周（n = 3）时处死动物。通过光学显微镜对活检样本进行组织形态计量学检查，并通过免疫组织化学染色测定血管内皮生长因子（VEGF）的表达。
在所有实验时间点，实验组中VEGF的免疫染色强度始终高于对照组。然而，在组织形态计量学和免疫组织化学检查中，对照组和实验组之间的差异无统计学意义。
本研究结果表明PRF可能会增加骨髓细胞数量。然而，PRF与异种骨替代物一起对骨再生未显示出显著影响。需要进一步的大规模研究来证实我们的结果。
