玉米黑粉菌铁载体的特性分析
Characterization of siderophores from Ustilago maydis.
作者信息
Budde A D, Leong S A
机构信息
USDA/ARS, Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin, Madison 53706.
出版信息
Mycopathologia. 1989 Nov;108(2):125-33. doi: 10.1007/BF00436063.
Two siderophores, ferrichrome and ferrichrome A, were found in cultures of Ustilago maydis (DC) Corda. Both siderophores were found intracellularly and extracellularly. Their authenticity was confirmed by thin layer chromatography, HPLC, UV-visible spectrometry, paper electrophoresis, amino acid analysis, NMR and fast atom bombardment mass spectroscopy. Regulation of siderophore production by iron was examined. Repression of biosynthesis of extracellular siderophores occurred at 10(-5) M iron. Ferrichrome was found intracellularly at all iron concentrations employed; in general, ferrichrome A was not found to be cell-associated.
在玉蜀黍黑粉菌（Ustilago maydis (DC) Corda）培养物中发现了两种铁载体，即铁色素和铁色素A。这两种铁载体在细胞内和细胞外均有发现。通过薄层色谱法、高效液相色谱法、紫外可见光谱法、纸电泳法、氨基酸分析、核磁共振和快原子轰击质谱法证实了它们的真实性。研究了铁对铁载体产生的调节作用。在铁浓度为10^(-5) M时，细胞外铁载体的生物合成受到抑制。在所使用的所有铁浓度下，铁色素都存在于细胞内；一般来说，未发现铁色素A与细胞相关。
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