Li Wen-Ying, Chen Xiao-Mei, Xiong Wei, Guo Dong-Mei, Lu Li, Li Hui-Yu
Center for Stem Cell Research and Application, Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Neonatal Screening Center, Zhuzhou Women and Children Medical Care Center, Zhuzhou, 412000, China.
J Huazhong Univ Sci Technolog Med Sci. 2014 Oct;34(5):640-645. doi: 10.1007/s11596-014-1330-0. Epub 2014 Oct 16.
Microvesicles (MVs) are the heterogeneous mixtures of vesicles. MVs released by leukemia cells constitute an important part of the leukemia microenvironment. MVs might act as important reservoirs of microRNAs (miRNAs). It is worth evaluating whether MVs possess some unique miRNA contents that are valuable in understanding the pathogenesis. In this study, we investigated the miRNA expression patterns of Nalm-6-derived MVs, Jurkat-derived MVs and normal cell-derived MVs using miRNA microarrays. The potential target genes regulated by differentially expressed miRNAs were also predicted and analyzed. Results demonstrated that 182 miRNAs and 166 miRNAs were differentially expressed in Nalm-6-MVs and Jurkat-MVs, respectively. Many oncogenes, tumor suppressors and signal pathway genes were targeted by these aberrantly expressed miRNAs, which might contribute to the development of B-ALL or T-ALL. Our findings expanded the potential diagnostic markers of ALL and provided useful information for ALL pathogenesis.
微泡（MVs）是囊泡的异质混合物。白血病细胞释放的MVs构成白血病微环境的重要组成部分。MVs可能作为微小RNA（miRNAs）的重要储存库。评估MVs是否具有一些在理解发病机制方面有价值的独特miRNA含量是值得的。在本研究中，我们使用miRNA微阵列研究了源自Nalm-6的MVs、源自Jurkat的MVs和源自正常细胞的MVs的miRNA表达模式。还对差异表达的miRNAs调控的潜在靶基因进行了预测和分析。结果表明，分别有182个miRNAs和166个miRNAs在Nalm-6-MVs和Jurkat-MVs中差异表达。许多癌基因、肿瘤抑制因子和信号通路基因被这些异常表达的miRNAs靶向，这可能有助于B-ALL或T-ALL的发展。我们的发现扩展了ALL的潜在诊断标志物，并为ALL发病机制提供了有用信息。
