Burke John G, Vettorato Enzo, Schöffmann Gudrun, Clutton R Eddie, Drew Tim S, Gibson J N Alastair
The Galway Clinic, Doughiska, Ireland.
Eur Spine J. 2015 Jul;24(7):1382-90. doi: 10.1007/s00586-014-3609-z. Epub 2014 Oct 16.
To characterise changes in vertebral dimension in an ovine model of scoliosis and determine whether a reproducible curvature could be created that would be suitable for future testing of curve modifying surgical implants.
At a mean age of 5 weeks, 28 Scottish blackface sheep were anaesthetised. A 4 mm braided synthetic tape was laid under the left lamina of T5 and L1 and tightened to 'hand' tension. A scoliosis was then created by binding the six lowest ribs on the same side just distal to their rib angles and resecting a segment from each of the opposite ribs. Radiographs were taken at 4 weekly intervals, and CT images at 2, 5 and 7 months post tethering, to determine multi-planar curve progression. 20 animals were assessed at age 3 months, 12 at 41 weeks and 10 at 1 year with comparisons to five control animals.
A significant bi-planar deformity was produced in all animals (scoliosis 32 ± 13º and lordosis 53 ± 21º 2 months post tethering; mean ± SD, n = 22). During the next 5 months the scoliosis decreased slightly (p = 0.08) but the sagittal deformity remained static: 21 ± 18° and 53 ± 19°, respectively (n = 12). The values at 7 months were associated with a mean 6 ± 4º rotational deformity. There was approximately twice as much growth in the right anterior aspects of the apical vertebrae as in the left posterior.
With appropriate care it was possible to obtain a reproducible curvature in immature sheep. The methods described are suitable for use in studies of growth modulation and other spinal devices.
在绵羊脊柱侧弯模型中表征椎体尺寸的变化，并确定是否可以创建可重复的弯曲，以适合未来对矫正脊柱弯曲的手术植入物进行测试。
在平均年龄为5周时，对28只苏格兰黑脸绵羊进行麻醉。将一条4毫米编织合成带置于T5和L1左侧椎板下方，并拉紧至“手”张力。然后通过在同一侧最低的六根肋骨的肋角远端进行绑扎，并从相对的每根肋骨上切除一段，来造成脊柱侧弯。每隔4周拍摄X线片，并在系绳后2、5和7个月拍摄CT图像，以确定多平面弯曲进展情况。在3个月龄时评估20只动物，41周龄时评估12只，1岁时评估10只，并与5只对照动物进行比较。
所有动物均产生了明显的双平面畸形（系绳后2个月脊柱侧弯32±13°，脊柱前凸53±21°；平均值±标准差，n = 22）。在接下来的5个月中，脊柱侧弯略有下降（p = 0.08），但矢状面畸形保持不变：分别为21±18°和53±19°（n = 12）。7个月时的值与平均6±4°的旋转畸形相关。顶椎右侧前部的生长量约为左侧后部的两倍。
通过适当的护理，可以在未成熟绵羊中获得可重复的弯曲。所描述的方法适用于生长调节和其他脊柱装置的研究。
