Lu Wei-Wei, Hou Ling-Ling, Zhang Wen-Wen, Zhang Peng-Fei, Chen Wen, Kang Xiangtao, Huang Yanqun
a College of Livestock Husbandry and Veterinary Engineering , Henan Agricultural University , Zhengzhou , Henan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2303-9. doi: 10.3109/19401736.2014.971022. Epub 2014 Oct 16.
NADH dehydrogenase subunit 2 gene (ND2) is one of the mitochondrial DNA (mtDNA) protein coding genes, which is a subunit of NADH dehydrogenase. The main purpose of this study was to investigate the variation/heteroplasmic sites of chicken ND2, and thus to evaluate the association with chicken growth traits, carcass traits, and serum biochemical indexes. Seventeen variants were detected in the ND2 gene by Sanger sequencing, which constructed 15 haplotypes; the haplotype diversity (hd) was 0.7692. Mt.A5703T and mt.T5727G in the ND2 gene had been detected as the heteroplasmic sites via the created restriction site restriction fragment length polymorphism (CRS-PCR-RFLP) method. Moreover, the study on distribution of two heteroplasmic variants in the Gushi chicken F2 resource population revealed that the heteroplasmic ratio of mt.A5703T and mt.T5727G was 9% and 40%, respectively. It showed that there was obvious heteroplasmic difference between two sites. Association analysis of the variation/heteroplasmy with the related traits in Gushi chicken F2 population showed that the mt.A5703T and mt.T5727G were significantly associated with the pectoral muscle fat content and the duodenum length, but no significance was found with body weight (BW). It was the first time to indicate that heteroplasmic variation had significant effect on growth traits, carcass parameters, and meat quality traits, which showed the potential importance of related variation.
NADH脱氢酶亚基2基因（ND2）是线粒体DNA（mtDNA）的蛋白质编码基因之一，是NADH脱氢酶的一个亚基。本研究的主要目的是调查鸡ND2基因的变异/异质性位点，从而评估其与鸡生长性状、胴体性状和血清生化指标之间 的关联。通过桑格测序在ND2基因中检测到17个变异，构建了15种单倍型；单倍型多样性（hd）为0.7692。通过创建限制性酶切位点限制性片段长度多态性（CRS-PCR-RFLP）方法，已将ND2基因中的mt.A5703T和mt.T5727G检测为异质性位点。此外，对固始鸡F2资源群体中两个异质性变异分布的研究表明，mt.A5703T和mt.T5727G的异质性比例分别为9%和40%。结果表明，两个位点之间存在明显的异质性差异。对固始鸡F2群体中变异/异质性与相关性状的关联分析表明，mt.A5703T和mt.T5727G与胸肌脂肪含量和十二指肠长度显著相关，但与体重（BW）无显著相关性。首次表明异质性变异对生长性状、胴体参数和肉质性状有显著影响，显示了相关变异的潜在重要性。
