Shi Jian, Guo Yadong, Zhang Chaoyue, Zhou Yong, Yan Jie, Liao Huidan, Lagabaiyila Zha
a The Third Xiangya Hospital, Central South University , Changsha , Hunan , P.R. China and.
b Department of Forensic Sciences , School of Basic Medicine, Central South University , Changsha , Hunan , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1910-1. doi: 10.3109/19401736.2014.971282. Epub 2014 Oct 16.
The complete mitochondrial genome of Parasarcophaga portschinskyi (Diptera: Sarcophagidae), a forensically important entomology was sequenced for the first time. The 14,929 bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes. It also contains one non-coding A + T-rich region. All the protein initiation codons are ATN, except for cox1 that begins with TCG. Each of the base composition on heavy strand was as follows A: 38.94%, G: 9.69%, C: 14.13%, T: 37.24% and the A + T content 76.18%. The mitochondrial genome of Parasarcophaga portschinskyi presented will be valuable for resolving phylogenetic relationships within the family Sarcophagidae and order Diptera, and could be used to identify favorable genetic markers for species identifications for forensic purposes.
首次对法医重要昆虫类的波特氏麻蝇（双翅目：麻蝇科）的完整线粒体基因组进行了测序。这个14,929 bp的环状基因组包含典型后生动物基因组中的37个基因：13个蛋白质编码基因、2个核糖体RNA基因和22个转运RNA基因。它还包含一个非编码的富含A+T的区域。除了以TCG起始的cox1外，所有蛋白质起始密码子均为ATN。重链上的每个碱基组成如下：A：38.94%，G：9.69%，C：14.13%，T：37.24%，A+T含量为76.18%。所呈现的波特氏麻蝇线粒体基因组对于解决麻蝇科和双翅目内的系统发育关系将具有重要价值，并且可用于鉴定法医目的物种识别的有利遗传标记。
