蚤蝇（Megaselia scalaris，双翅目：蚤蝇科）的完整线粒体基因组
The complete mitochondrial genome of the scuttle fly, Megaselia scalaris (Diptera: Phoridae).
作者信息
Zhong Ming, Wang Xiang, Liu Qinlai, Luo Baihua, Wu Chang, Wen Jifang
机构信息
a Department of Pathology , School of Basic Medical Sciences, Central South University , Changsha , Hunan , China .
b Institute of Cancer Stem Cell, Cancer Center, Dalian Medical University , Dalian , Liaoning , P.R. China , and.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):182-4. doi: 10.3109/19401736.2013.879651. Epub 2014 Feb 3.
More than 1400 scuttle flies species in worldwide comprise the Megaselia genus, the largest genus in the family Phoridae. The complete mitochondrial genome of Megaselia scalaris, a medically important entomology was sequenced for the first time. The 15,599 bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The mitochondrial genome also contains one non-coding A + T-rich region. The arrangement of the genes was identical with other insect. Each of the base composition on heavy strand was as follows A: 38.87%, G: 13.74%, C: 9.46%, T: 37.93% and the A + T content 76.80%. The mitochondrial genome of M. scalaris presented may be valuable for determining phylogenetic relationships within the order Diptera and especially for the family Phoridae. These sequences could also be used to select reliable molecular markers for species identification in forensic entomology.
全球有1400多种蚤蝇属于Megaselia属，是蚤蝇科中最大的属。首次对一种具有重要医学意义的昆虫——厩腐蝇的完整线粒体基因组进行了测序。这个15599碱基对的环状基因组包含了典型后生动物基因组中的37个基因：13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因。线粒体基因组还包含一个非编码的富含A+T的区域。这些基因的排列与其他昆虫相同。重链上的碱基组成分别为：A：38.87%，G：13.74%，C：9.46%，T：37.93%，A+T含量为76.80%。所呈现的厩腐蝇线粒体基因组可能对于确定双翅目内部的系统发育关系，特别是对于蚤蝇科而言具有重要价值。这些序列也可用于在法医昆虫学中选择可靠的分子标记进行物种鉴定。
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