Lan Lingmei, Liu Ying, Yan Jie, Lin Lin, Zha Lagabaiyila
a Department of Forensic Sciences , School of Basic Medicine, Central South University , Changsha , Hunan , China and.
b Department of Oral and Maxillofacial Surgery , Xiangya Stomatological Hospital, Central South University , Changsha , Hunan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4069-4070. doi: 10.3109/19401736.2014.1003847. Epub 2015 Jan 28.
In this study, we sequence the complete mitochondrial genome of Muscina stabulans (Diptera: Muscidae), a forensically important entomology, for the first time. The 15,933 bp circular genome contains the 37 genes found in a typical metazoan genome: 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding A + T-rich region. All the protein initiation codons are ATN, except for cox1 and ND1 that begin with TCG and TTG. The arrangement of the genes was the same as that found in the other insect. The overall base composition on heavy strand was as follows A: 40.00%, G: 8.80%, C: 13.34%, T: 37.86% and the A + T content 77.86%. The mitochondrial genome of Muscina stabulans presented will be valuable for resolving phylogenetic relationships within the family Muscidae and order Diptera, and could be used to identify favourable genetic markers for species identifications for forensic purposes.
在本研究中，我们首次对法医重要昆虫厩螫蝇（双翅目：蝇科）的完整线粒体基因组进行了测序。这个15933bp的环状基因组包含了典型后生动物基因组中的37个基因：13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和1个富含A+T的非编码区。除了以TCG和TTG起始的cox1和ND1外，所有蛋白质起始密码子均为ATN。基因排列与其他昆虫相同。重链上的总体碱基组成为：A：40.00%，G：8.80%，C：13.34%，T：37.86%，A+T含量为77.86%。所呈现的厩螫蝇线粒体基因组对于解决蝇科和双翅目内的系统发育关系将具有重要价值，并可用于鉴定法医物种鉴定的有利遗传标记。
