Li Fengbo, Zhang Huixian, Liu Peigang, Wang Yongqiang, Meng Zhiqi
a State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control , Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences , Hangzhou , P.R. China .
b College of Chemistry and Life Sciences, Zhejiang Normal University , Jinhua , P.R. China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1844-5. doi: 10.3109/19401736.2014.971252. Epub 2014 Oct 16.
The hybrid strain of the domesticated silkworm (Qiufeng × Baiyu) is one of the most popular commercial silkworm varieties in China. In this study, we reported its complete mitochondrial genome sequence for the first time. The 15,680 bp long genome contains 37 genes (13 protein-coding genes [PCGs], 2 rRNA genes, and 22 tRNA genes) and 1 major non-coding A + T-rich region, with the typical arrangement found in Lepidoptera. All PCGs started with typical ATN codons except for COI, which began with CGA. Eleven PCGs have complete stop codons, whereas COI and COII end with a single T. The 495 bp long A + T-rich region harbors the conserved sequence features typically found in lepidopteran insects. The complete mitochondrial genome sequence of Qiufeng × Baiyu provides an important data source for further study on the mechanism of silkworm domestication.
家蚕杂交品种(秋丰×白玉)是中国最受欢迎的商业蚕品种之一。在本研究中,我们首次报道了其完整的线粒体基因组序列。该基因组长度为15,680 bp,包含37个基因(13个蛋白质编码基因[PCGs]、2个rRNA基因和22个tRNA基因)以及1个主要的非编码富含A+T区域,具有鳞翅目昆虫中典型的排列方式。除COI以CGA起始外,所有PCGs均以典型的ATN密码子起始。11个PCGs具有完整的终止密码子,而COI和COII以单个T结尾。长度为495 bp的富含A+T区域具有鳞翅目昆虫中通常发现的保守序列特征。秋丰×白玉的完整线粒体基因组序列为进一步研究家蚕驯化机制提供了重要的数据来源。