Sacco R L, Owen J, Mohr J P, Tatemichi T K, Grossman B A
Neurological Institute, New York, NY 10032.
Stroke. 1989 Dec;20(12):1657-61. doi: 10.1161/01.str.20.12.1657.
A deficiency of free protein S, known to increase the risk of peripheral venous thrombosis, has not been well described in patients with cerebrovascular disease. In a pilot study of 35 patients with symptomatic cerebrovascular disease, using a qualitative crossed immunoelectrophoresis assay we found eight patients with a free protein S deficiency. A Laurell immunoelectrophoresis assay was used to quantify the percentage of free protein S after removal of the inactive protein S C4b-binding protein complex by precipitation with polyethylene glycol 8000. In a quantitative study of 103 patients with cerebrovascular disease, 21 had a free protein S that was less than 20% of the average normal total protein S concentration (normal range 20-40%); 19 had suffered cerebral infarction and the other two had suffered intracranial hemorrhage. The frequency of free protein S deficiency in this group of stroke patients was far higher than the expected prevalence in the general population.
已知游离蛋白S缺乏会增加外周静脉血栓形成的风险，但在脑血管疾病患者中对此尚未有充分描述。在一项针对35例有症状脑血管疾病患者的初步研究中，我们采用定性交叉免疫电泳分析法，发现8例患者存在游离蛋白S缺乏。使用劳雷尔免疫电泳分析法，通过用聚乙二醇8000沉淀去除无活性的蛋白S-C4b结合蛋白复合物后，对游离蛋白S的百分比进行定量。在一项针对103例脑血管疾病患者的定量研究中，21例患者的游离蛋白S低于正常总蛋白S平均浓度的20%（正常范围为20%-40%）；其中19例发生脑梗死，另外2例发生颅内出血。这组中风患者中游离蛋白S缺乏的频率远高于普通人群的预期患病率。
