Raich Teresa, Powell Scott
Nanosphere Inc., 4088 Commercial Avenue, Northbrook, 60062, IL, USA.
Methods Mol Biol. 2015;1237:73-90. doi: 10.1007/978-1-4939-1776-1_8.
Rapid identification and characterization of bacterial and fungal pathogens present in the bloodstream are essential for optimal patient management and are associated with improved patient outcomes, improved antimicrobial stewardship, improved infection control, and reduced healthcare costs. Microarrays serve as reliable platforms for the identification of these bloodstream pathogens and their associated antimicrobial resistance genes, if present. Nanosphere's (Nanosphere, Inc., Northbrook, IL, USA) Verigene Gram-Positive Blood Culture Nucleic-Acid Test (BC-GP) is one such microarray-based approach for the detection of bacteria that cause bloodstream infection. Here, we describe the design of the microarray-based Verigene BC-GP Test, the steps necessary for performing the test, and the different components of the test including nucleic acid extraction and hybridization of target nucleic acid to a microarray.
快速鉴定和表征血流中存在的细菌和真菌病原体对于患者的最佳管理至关重要，并且与改善患者预后、加强抗菌药物管理、改善感染控制以及降低医疗成本相关。微阵列是用于鉴定这些血流病原体及其相关抗菌耐药基因（如果存在）的可靠平台。美国伊利诺伊州北布鲁克的Nanosphere公司的Verigene革兰氏阳性血培养核酸检测（BC-GP）就是这样一种基于微阵列的检测引起血流感染细菌的方法。在此，我们描述基于微阵列的Verigene BC-GP检测的设计、进行该检测所需的步骤以及该检测的不同组成部分，包括核酸提取和靶核酸与微阵列的杂交。
