Hrabák Jaroslav
Department of Microbiology, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Alej Svobody 80, 304 60, Plzen, Czech Republic,
Methods Mol Biol. 2015;1237:91-6. doi: 10.1007/978-1-4939-1776-1_9.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identification of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confirm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）最近已被引入许多诊断微生物实验室。除了用于鉴定细菌和真菌外，该技术还为检测抗菌药物耐药性，特别是由β-内酰胺酶介导的耐药性，提供了一种潜在的有用工具。在此，我们描述了一种使用MALDI-TOF MS检测肠杆菌科、假单胞菌属和鲍曼不动杆菌临床分离株中美罗培南水解的测定方法。该方法能够在3小时内确认碳青霉烯酶。这些结果对于采取适当且快速的干预措施以限制产碳青霉烯酶细菌的传播非常重要，并为这些微生物引起的感染的适当初始治疗提供信息。
