Elbehiry Ayman, Aldubaib Musaad, Abalkhail Adil, Marzouk Eman, ALbeloushi Ahmad, Moussa Ihab, Ibrahem Mai, Albazie Hamad, Alqarni Abdullah, Anagreyyah Sulaiman, Alghamdi Saleh, Rawway Mohammed
Department of Public Health, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah 52741, Saudi Arabia.
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City 32511, Egypt.
Vaccines (Basel). 2022 Nov 8;10(11):1881. doi: 10.3390/vaccines10111881.
Healthcare settings have been utilizing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) since 2010. MALDI-TOF MS has various benefits over the conventional method of biochemical identification, including ease of use, speed, accuracy, and low cost. This approach can solve many of the obstacles to identifying bacteria, fungi and viruses. As technology advanced, more and more databases kept track of spectra, allowing species with similar morphological, genotypic, and biochemical traits to be identified. Using MALDI-TOF MS for identification has become more accurate and quicker due to advances in sample preparation and database enrichment. Rapid sample detection and colony identification using MALDI-TOF MS have produced promising results. A key application of MALDI-TOF MS is quickly identifying highly virulent and drug-resistant diseases. Here, we present a review of the scientific literature assessing the effectiveness of MALDI-TOF MS for locating clinically relevant pathogenic bacteria, fungi, and viruses. MALDI-TOF MS is a useful strategy for locating clinical pathogens, however, it also has some drawbacks. A small number of spectra in the database and inherent similarities among organisms can make it difficult to distinguish between different species, which can result in misidentifications. The majority of the time additional testing may correct these problems, which happen very seldom. In conclusion, infectious illness diagnosis and clinical care are being revolutionized by the use of MALDI-TOF MS in the clinical microbiology laboratory.
自2010年以来，医疗机构一直在使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）。与传统的生化鉴定方法相比，MALDI-TOF MS具有多种优势，包括易用性、速度、准确性和低成本。这种方法可以解决细菌、真菌和病毒鉴定中的许多障碍。随着技术的进步，越来越多的数据库记录了光谱，从而能够识别具有相似形态、基因型和生化特征的物种。由于样品制备和数据库丰富方面的进展，使用MALDI-TOF MS进行鉴定变得更加准确和快捷。使用MALDI-TOF MS进行快速样品检测和菌落鉴定已经取得了令人鼓舞的结果。MALDI-TOF MS的一个关键应用是快速识别高毒力和耐药性疾病。在此，我们对评估MALDI-TOF MS在定位临床相关致病细菌、真菌和病毒方面有效性的科学文献进行综述。MALDI-TOF MS是定位临床病原体的一种有用策略，然而，它也有一些缺点。数据库中的光谱数量较少以及生物体之间固有的相似性可能导致难以区分不同物种，从而可能导致错误鉴定。大多数情况下，额外的检测可能会纠正这些很少发生的问题。总之，临床微生物实验室中使用MALDI-TOF MS正在彻底改变传染病诊断和临床护理。
